MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells

  • Authors:
    • Naotake Funamizu
    • Curtis R. Lacy
    • Minori Kamada
    • Katsuhiko Yanaga
    • Yoshinobu Manome
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  • Published online on: September 22, 2015     https://doi.org/10.3892/ijo.2015.3178
  • Pages: 1981-1988
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Abstract

The present study investigated the relationship between microRNA-203 (miR-203) and the p53 upregulated modulator of apoptosis (Puma) in colon (HCT116) and lung cancer (A549) cells. Colon and lung cancer cell lines were selected for this study since a relationship between p53/miR-203 and p53/Puma has been established in both cancers. In the present study, adriamycin and nutlin-3 were used to activate p53, which induced both miR-203 and Puma expression in HCT116 cells. In contrast, HCT 116 cells with downregulated p53 showed decreased miR-203 and Puma expression. Importantly, we found that overexpressed miR-203 in HCT116 cells resulted in significantly increased Puma expression (P<0.05). Based on these findings, we hypothesized that another limb of the p53/Puma axis depends on miR-203 expression. To further validate this relationship, we used lung cancer cells (A549) and found that activated p53 increased both miR-203 and Puma expression. In addition, we found that Puma expression remained elevated in cells with overexpressed miR-203 in the presence of p53 downregulation. Cumulatively, our data purport that p53 not only increased Puma expression directly, but that it may also do so through miR-203. Additionally, functional studies revealed that miR-203 overexpression induced apoptosis and inhibited cell invasiveness.
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November-2015
Volume 47 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Funamizu N, Lacy CR, Kamada M, Yanaga K and Manome Y: MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells. Int J Oncol 47: 1981-1988, 2015
APA
Funamizu, N., Lacy, C.R., Kamada, M., Yanaga, K., & Manome, Y. (2015). MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells. International Journal of Oncology, 47, 1981-1988. https://doi.org/10.3892/ijo.2015.3178
MLA
Funamizu, N., Lacy, C. R., Kamada, M., Yanaga, K., Manome, Y."MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells". International Journal of Oncology 47.5 (2015): 1981-1988.
Chicago
Funamizu, N., Lacy, C. R., Kamada, M., Yanaga, K., Manome, Y."MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells". International Journal of Oncology 47, no. 5 (2015): 1981-1988. https://doi.org/10.3892/ijo.2015.3178