Differential expression of Oct3/4 in human breast cancer and normal tissues

  • Authors:
    • Feng-Qi Zhao
    • Yogi Misra
    • Da-Biao Li
    • Marilyn P. Wadsworth
    • David Krag
    • Donald Weaver
    • Joseph Tessitore
    • Da-Wei Li
    • Guo Zhang
    • Qing Tian
    • Katie Buss
  • View Affiliations

  • Published online on: March 29, 2018     https://doi.org/10.3892/ijo.2018.4341
  • Pages: 2069-2078
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Abstract

Oct3/4, a transcription factor specifically expressed in mammalian totipotent embryonic stem and germ cells, has a critical role in the regulation and maintenance of pluripotency and self-renewal. However, reactivation of Oct3/4 expression is observed in several human breast cancer cell lines, but not in non‑malignant cells. To examine Oct3/4 expression in human primary breast carcinomas and normal breast tissues, we obtained breast tumor tissues from 28 patients and normal breast tissues from 9 women. According to quantitative polymerase chain reaction, all of the tumor tissues, irrespective of tumor type or clinicopathological status, expressed Oct3/4 mRNA at 10- to 100- fold higher levels than that in the normal breast tissues. Expression of the Oct3/4 protein in tumors was confirmed by western blot analysis and immunofluorescent staining. Additionally, rapid amplification of cDNA ends and DNA sequencing revealed expression of multiple Oct4 gene transcripts from chromosome 6 (POU5F1) in normal breast tissues and the non‑malignant breast epithelial cell line MCF‑10A; by contrast, the breast tumors and malignant breast cancer cell line MCF‑7 predominantly expressed transcripts of an Oct4-like gene (POU5F1B) from chromosome 8, which was termed Oct3 in the current study. The deduced amino acid sequences of full-length Oct3 and Oct4 are 96% identical. The findings of the current study indicated that Oct3, rather than Oct4, may serve as a novel clinical marker and a potential target for gene-specific therapy of breast cancer.
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June-2018
Volume 52 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhao F, Misra Y, Li D, Wadsworth MP, Krag D, Weaver D, Tessitore J, Li D, Zhang G, Tian Q, Tian Q, et al: Differential expression of Oct3/4 in human breast cancer and normal tissues. Int J Oncol 52: 2069-2078, 2018
APA
Zhao, F., Misra, Y., Li, D., Wadsworth, M.P., Krag, D., Weaver, D. ... Buss, K. (2018). Differential expression of Oct3/4 in human breast cancer and normal tissues. International Journal of Oncology, 52, 2069-2078. https://doi.org/10.3892/ijo.2018.4341
MLA
Zhao, F., Misra, Y., Li, D., Wadsworth, M. P., Krag, D., Weaver, D., Tessitore, J., Li, D., Zhang, G., Tian, Q., Buss, K."Differential expression of Oct3/4 in human breast cancer and normal tissues". International Journal of Oncology 52.6 (2018): 2069-2078.
Chicago
Zhao, F., Misra, Y., Li, D., Wadsworth, M. P., Krag, D., Weaver, D., Tessitore, J., Li, D., Zhang, G., Tian, Q., Buss, K."Differential expression of Oct3/4 in human breast cancer and normal tissues". International Journal of Oncology 52, no. 6 (2018): 2069-2078. https://doi.org/10.3892/ijo.2018.4341