Rituximab used in three cases with relapsed non‑Hodgkin's lymphoma

  • Authors:
    • Murat Elli
    • Sema Yilmaz
    • Ramazan Aydin
    • Sadriye Murat
    • Meltem Ceyhan Bilgici
    • Ayhan Dagdemir
  • View Affiliations

  • Published online on: March 5, 2013     https://doi.org/10.3892/mco.2013.88
  • Pages: 550-552
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Relapsed or refractory B‑cell non‑Hodgkin's lymphoma (B-NHL) patients have a poor prognosis. New treatment modalities have been used to improve survival rates in children with relapsed or refractory B-NHL. CD20 is expressed in >98% of childhood B-NHL and a chimeric anti‑CD20 monoclonal antibody, rituximab, is increasingly being used at relapse. The aim of the present study was to determine the efficacy of rituximab on relapsed B-NHL. Three B-NHL cases were treated successfully with a combination of intensive chemotherapy protocol plus rituximab.

Introduction

Lymphomas are the third most common type of cancer identified during childhood and adolescence, with 60% of those being mature B-cell non-Hodgkin’s lymphoma (B-NHL). Primary central nervous system malignant lymphomas (PCNSLs) comprise <2% of malignant lymphomas. B-NHL is usually of B-cell origin (14). First-line chemotherapy is found to be effective in the majority of children diagnosed with B-NHL. Although long-term cure rates are 75% for high-risk disease (5), relapses occur in ∼20% of the patients, almost always within a year from diagnosis (6). Relapsed or refractory B-NHL has a poor prognosis. CD20 is expressed in >98% of childhood B-NHL and increasingly a chimeric anti-CD20 monoclonal antibody, rituximab, is being used at relapse (7,8). Although rituximab is commonly used as a first-line therapy in adults, the effect of rituximab in children with B-NHL has yet to be adequately investigated (912). Three B-NHL cases were investigated in this case study to determine the efficacy of rituximab-containing regimens on relapsed B-NHL.

Case reports

Patient 1

A 16-year-old male was admitted to the Department of Pediatric Oncology complaining of recurrent abdominal pain and distention for the previous 2 months. Physical examination revealed that the abdomen was distended but non-tender, with an immobile, painless and hard mass, 5×8 cm in diameter, located on the right side of the abdomen. Results of bone marrow (BM) examination, hematologic and basic metabolic analysis were normal. Abdominal computed tomography (CT) scan confirmed multiple retroperitoneal lymph nodes and a mass, 6×10×11 cm in a diameter, surrounding the superior mesenteric artery (Fig. 1A). Burkitt lymphoma was diagnosed. Positive CD20, CD10, bcl6 and negative CD30, CD3, CD5 and TdT were reported by immunohistochemical examination. After 4 months of NHL-Berlin-Frankfurt-Muenster (BFM) 95 chemotherapy protocol, the patient noted backache. BM examination revealed diffuse L3 type lymphoblasts. Additionally, the CT scan showed that the size of the intra-abdominal mass did not decrease. The patient received intravenous rituximab (375 mg/m2/dose/once every 3 weeks) for six doses and Ifosfamide, Carboplatin and Etoposide (ICE). The control CT scan revealed that the size of the mass decreased and central necrosis was evident (Fig. 1B). Although in complete remission at least 12 months following chemotherapy, the patient succumbed due to systemic progression of severe sepsis.

Patient 2

A 14-year-old male presented with a 1-month history of abdominal pain and distension. He noted a 7-pound weight loss during the previous two weeks. On physical examination, the abdomen was distended, although soft and non-tender, with hypoactive bowel sounds and shifting dullness. No organomegaly was noted. Results of BM examination, as well as hematologic and metabolic tests were normal. Abdominal CT scan revealed enlarged multiple lymphadenopathies, of which the largest was 6 cm in diameter, in the mesenteric region and peritoneal surfaces with ascites (Fig. 1C). Flow cytometry showed that the ascites fluid was 90% positive for CD45 and 80% for CD20. Burkitt lymphoma was diagnosed and NHL-BFM 95 chemotherapy protocol was administered. After 6 months of chemotherapy, the abdominal and the maxillary mass gradually decreased. At the end of the chemotherapy, complete remission was achieved (Fig. 1D). One month later, the patient complained of chest pain and a 4×3 cm solid mass on the right 4th rib was detected in the thorax CT scan. Fine-needle aspiration confirmed B-cell lymphoma. ICE and intravenous rituximab (375 mg/m2/dose/once every 3 weeks) for 6 weeks were given and remission was achieved again. After 2 years of follow-up, the patient is still in complete remission.

Patient 3

A previously healthy 4.5-year-old girl was admitted with a 1-month history of headache and seizures. Her physical examination was normal. BM examination, routine blood chemistry and hematologic parameters had no abnormality. The CT revealed multiple homogeneously enhancing dural mass lesions (Fig. 1E). There was no evidence of lymphoma in any other anatomic location. After partial excision, immunohistochemistry revealed highly expressed HLA-DR, CD19, CD20, CD38 and CD79a. B-NHL was diagnosed. The patient was administered intensive chemotherapy with NHL-BFM 95 chemotherapy protocol. However, at the fourth month of treatment, right maxillary swelling was identified. The mass, 4×3×5 cm, was evident on maxillofacial MRI scan. Diffuse L3 type lymphoblasts were observed with fine needle aspiration. Two doses of rituximab (375 mg/m2/dose/once every 3 weeks) were given. The control CT scan revealed that the cranial tumor completely regressed (Fig. 1F). However, the patient’s status deteriorated gradually due to sepsis. Consequently acute respiratory distress syndrome and multi-organ failure developed. The patient ultimately succumbed one year after the initiation of chemotherapy of causes unrelated to central nervous system lymphoma (CNSL).

Discussion

Although an improvement in the treatment outcome for children with high-grade B-cell lymphomas has been noted, the prognosis of relapsed or refractory B-cell lymphomas is poor (10). Rituximab has shown good clinical activity in the treatment of CD20-positive B-cell lymphomas. It has also been reported that rituximab provides the opportunity for combining to chemotherapy combinations and increasing the overall and disease-free survival (1115). Our first case received rituximab in addition to ICE after relapsed B-NHL was diagnosed. One month after completion of the chemotherapy, the tumor size was found to have markedly decreased and there were no blasts in BM. This case supports a potential role of rituximab in children with established relapsed or refractory B-NHL.

The Children’s Oncology Group reported that the toxicity of combination with ICE and rituximab was acceptable in children with recurrent or refractory B-NHL (10). Rituximab in combination with ICE was well tolerated and proved to be effective, with no major side effects. The abdominal mass completely recovered with NHL-BFM 95 chemotherapy protocol in the second case. Following rapid detection of a new mass on the patient’s right 4th rib, rituximab in combination with ICE was administered and complete remission was achieved. The patient remains in complete remission after 2 years of completing combined therapy. Taken together, for patients who do not achieve complete remission or relapse following reinduction chemotherapy, rituximab combined with intensive chemotherapy could provide a beneficial role in the treatment of B-NHL. Although the number of patients in this study was limited, the follow-up of a larger group of patients could aid in determining the benefits of rituximab in children with B-NHL.

Large-group studies have reported that <1% of PCNSLs, either B- or T-cell, occur in patients under 18 years of age (4,11,16,17). Due to the insufficient number of prospective studies, it is not easy to determine the best therapeutic option for this rare tumor (18). Rituximab was found to be effective in adults with secondary CNSLs (19). When no remission was achieved, the cranial tumor almost completely recovered with rituximab in our case. Although we obtained a remission in only one patient, better understanding of rituximab in pediatric PCNSLs should be emphasized with large-scale studies. PCNSLs tended to occur more frequently in immunodeficient children (17,18). Our case was not immunosuppressive. Therefore, the proper time to apply rituximab is while being unresponsive to current pediatric protocols in relapsed/refractory B-NHL.

This case series has demonstrated that early treatment with rituximab alone or combined with intensive chemotherapy could play a significant role in improving the outcome of relapsed B-cell lymphomas in the pediatric age group. Nevertheless, the identification of clinical efficacy and safety of rituximab as a monotheraphy or combination chemotherapy should be investigated in a large pediatric series.

References

1. 

Bilić E, Femenić R, Konja J, et al: CD20 positive childhood B-non Hodgkin lymphoma (B-NHL): morphology, immunophenotype and a novel treatment approach: a single center experience. Coll Antropol. 34:171–175. 2010.PubMed/NCBI

2. 

Molyneux EM, Rochford R, Griffin B, et al: Burkitt’s lymphoma. Lancet. 379:1234–1244. 2012.

3. 

Akbayram S, Doğan M, Akgün C, et al: Use of rituximab in three children with relapsed/refractory Burkitt lymphoma. Target Oncol. 5:291–294. 2010. View Article : Google Scholar : PubMed/NCBI

4. 

Alasaad T and Barr R: Successful treatment of multiply relapsed lymphoma with rituximab as a single agent. Pediatr Blood Cancer. 55:356–358. 2010. View Article : Google Scholar : PubMed/NCBI

5. 

Attarbaschi A, Dworzak M, Steiner M, et al: Outcome of children with primary resistant or relapsed non-Hodgkin lymphoma and mature B-cell leukemia after intensive first-line treatment: a population-based analysis of the Austrian Cooperative Study Group. Pediatr Blood Cancer. 44:70–76. 2005. View Article : Google Scholar

6. 

Bay A, Dogan M, Acikgoz M and Oner AF: Rituximab in a child with relapsed Burkitt lymphoma. Pediatr Blood Cancer. 49:2182007. View Article : Google Scholar : PubMed/NCBI

7. 

Culić S, Culić V, Armanda V, et al: Anti-CD20 monoclonal antibody (Rituximab) for therapy of mediastinal CD20-positive large B-cell non-Hodgkin lymphoma with a local tumor extension into the lung of a 10-year-old girl. Pediatr Hematol Oncol. 20:339–344. 2003.PubMed/NCBI

8. 

Meinhardt A, Burkhardt B, Zimmermann M, et al: Phase II window study on rituximab in newly diagnosed pediatric mature B-cell non-Hodgkin’s lymphoma and Burkitt leukemia. J Clin Oncol. 28:3115–3121. 2010.PubMed/NCBI

9. 

Corbacioglu S, Eber S, Gungor T, et al: Induction of long-term remission of a relapsed childhood B-acute lymphoblastic leukemia with rituximab chimeric anti-CD20 monoclonal antibody and autologous stem cell transplantation. J Pediatr Hematol Oncol. 25:327–329. 2003. View Article : Google Scholar : PubMed/NCBI

10. 

Griffin TC, Weitzman S, Weinstein H, et al: A study of rituximab and ifosfamide, carboplatin, and etoposide chemotherapy in children with recurrent/refractory B-cell (CD20+) non-Hodgkin lymphoma and mature B-cell acute lymphoblastic leukemia: a report from the Children’s Oncology Group. Pediatr Blood Cancer. 52:177–181. 2009.PubMed/NCBI

11. 

Boyle EM and Morschhauser F: Ongoing development of monoclonal antibodies and antibody drug-conjugates in lymphoma. Curr Oncol Rep. 13:386–397. 2011. View Article : Google Scholar : PubMed/NCBI

12. 

Leget GA and Czuczman MS: Use of rituximab, the new FDA approved antibody. Curr Opin Oncol. 10:548–551. 1998. View Article : Google Scholar : PubMed/NCBI

13. 

Song KW, Mollee P, Patterson B, et al: Pure red cell aplasia due to parvovirus following treatment with CHOP and rituximab for B-cell lymphoma. Br J Haematol. 119:125–127. 2002. View Article : Google Scholar : PubMed/NCBI

14. 

Quartier P, Brethon B, Philippet P, et al: Treatment of childhood autoimmune haemolytic anaemia with rituximab. Lancet. 358:1511–1513. 2001. View Article : Google Scholar : PubMed/NCBI

15. 

Zecca M, Nobili B, Ramenghi U, et al: Rituximab for the treatment of refractory autoimmune hemolytic anemia in children. Blood. 101:3857–3861. 2003. View Article : Google Scholar : PubMed/NCBI

16. 

Campo E, Swerdlow SH, Harris NL, et al: The 2008 WHO classification of lymphoid neoplasms and beyond: evolving concepts and practical applications. Blood. 117:5019–5032. 2011. View Article : Google Scholar : PubMed/NCBI

17. 

Bhagavathi S and Wilson JD: Primary central nervous system lymphoma. Arch Pathol Lab Med. 132:1830–1834. 2008.PubMed/NCBI

18. 

Abla O and Weitzman S: Primary central nervous system lymphoma in children. Neurosurg Focus. 21:E82006. View Article : Google Scholar : PubMed/NCBI

19. 

Kawano N, Ochiai H, Yoshida S, et al: Clinical features and treatment outcomes of isolated secondary central nervous system lymphomas in Miyazaki Prefecture. Int J Clin Oncol. 17:336–340. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

Journal Cover

May-June 2013
Volume 1 Issue 3

Print ISSN: 2049-9450
Online ISSN:2049-9469

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Elli M, Yilmaz S, Aydin R, Murat S, Bilgici MC and Dagdemir A: Rituximab used in three cases with relapsed non‑Hodgkin's lymphoma. Mol Clin Oncol 1: 550-552, 2013
APA
Elli, M., Yilmaz, S., Aydin, R., Murat, S., Bilgici, M.C., & Dagdemir, A. (2013). Rituximab used in three cases with relapsed non‑Hodgkin's lymphoma. Molecular and Clinical Oncology, 1, 550-552. https://doi.org/10.3892/mco.2013.88
MLA
Elli, M., Yilmaz, S., Aydin, R., Murat, S., Bilgici, M. C., Dagdemir, A."Rituximab used in three cases with relapsed non‑Hodgkin's lymphoma". Molecular and Clinical Oncology 1.3 (2013): 550-552.
Chicago
Elli, M., Yilmaz, S., Aydin, R., Murat, S., Bilgici, M. C., Dagdemir, A."Rituximab used in three cases with relapsed non‑Hodgkin's lymphoma". Molecular and Clinical Oncology 1, no. 3 (2013): 550-552. https://doi.org/10.3892/mco.2013.88