Inducing human parthenogenetic embryonic stem cells into islet‑like clusters

Li,Jin; He,Jingjing; Lin,Ge; Lu,Guangxiu

doi:10.3892/mmr.2014.2588

Inducing human parthenogenetic embryonic stem cells into islet‑like clusters

Authors:
- Jin Li
- Jingjing He
- Ge Lin
- Guangxiu Lu
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Affiliations: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China
Published online on: September 22, 2014 https://doi.org/10.3892/mmr.2014.2588
Pages: 2882-2890
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

In order to determine whether human parthenogenetic embryonic stem (hpES) cells have the potential to differentiate into functional cells, a modified four‑step protocol was used to induce the hpES cells into islet‑like clusters (ILCs) in vitro. Growth factors activin A, retinoic acid, nicotinamide, Exendin‑4 and betacellulin were added sequentially to the hpES cells at each step. The terminally differentiated cells were shown to gather into ILCs. Immunohistochemistry and semi quantitative polymerase chain reaction analyses demonstrated that the ILCs expressed islet specific hormones and functional markers. Furthermore, an insulin release test indicated that the clusters had the same physiological function as islets. The ILCs derived from hpES cells shared similar characteristics with islets. These results indicate that hpES cell‑derived ILCs may be used as reliable material for the treatment of type I diabetes mellitus.

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