LY294002 suppresses the malignant phenotype and sensitizes osteosarcoma cells to pirarubicin chemotherapy

  • Authors:
    • Xin Hua Long
    • Zhen Hao Zhong
    • Ai Fen Peng
    • Liang  Bo Zhu
    • Heng Wang
    • Guo Mei Zhang
    • Zhi Li Liu
  • View Affiliations

  • Published online on: October 9, 2014     https://doi.org/10.3892/mmr.2014.2617
  • Pages: 2967-2972
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Abstract

Pirarubicin is frequently used in chemotherapy against tumors. However, clinical use is severely limited by the development of progressive dose-dependent cardiomyopathy and acquired drug resistance. LY294002 is a commonly used pharmacologic inhibitor, which selectively inhibits the phosphoinositide 3-kinase-AKT nexus. The aim of this study was to investigate the combined inhibitory effect of LY294002 and pirarubicin on human osteosarcoma (OS) cells in vitro. The inhibitory effect of LY294002 plus pirarubicin on U2-OS and MG-63 OS cell proliferation, apoptosis, migration and invasion was investigated by cell proliferation, wound healing and Transwell invasion assays. The results revealed that LY294002 and pirarubicin synergistically induced apoptosis, and inhibited the growth, migration and invasion of OS cells. This indicates that LY294002 enhanced the effects of pirarubicin on OS in vitro. LY294002 combined with pirarubicin may thus be a future therapeutic strategy in OS.
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December-2014
Volume 10 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Long XH, Zhong ZH, Peng AF, Zhu LB, Wang H, Zhang GM and Liu ZL: LY294002 suppresses the malignant phenotype and sensitizes osteosarcoma cells to pirarubicin chemotherapy. Mol Med Rep 10: 2967-2972, 2014
APA
Long, X.H., Zhong, Z.H., Peng, A.F., Zhu, L.B., Wang, H., Zhang, G.M., & Liu, Z.L. (2014). LY294002 suppresses the malignant phenotype and sensitizes osteosarcoma cells to pirarubicin chemotherapy. Molecular Medicine Reports, 10, 2967-2972. https://doi.org/10.3892/mmr.2014.2617
MLA
Long, X. H., Zhong, Z. H., Peng, A. F., Zhu, L. B., Wang, H., Zhang, G. M., Liu, Z. L."LY294002 suppresses the malignant phenotype and sensitizes osteosarcoma cells to pirarubicin chemotherapy". Molecular Medicine Reports 10.6 (2014): 2967-2972.
Chicago
Long, X. H., Zhong, Z. H., Peng, A. F., Zhu, L. B., Wang, H., Zhang, G. M., Liu, Z. L."LY294002 suppresses the malignant phenotype and sensitizes osteosarcoma cells to pirarubicin chemotherapy". Molecular Medicine Reports 10, no. 6 (2014): 2967-2972. https://doi.org/10.3892/mmr.2014.2617