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In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction

  • Authors:
    • Ping Hua
    • You‑Yu Wang
    • Li‑Bao Liu
    • Jia‑Liang Liu
    • Jian‑Yang Liu
    • Yan‑Qi Yang
    • Song‑Ran Yang

  • View Affiliations

  • Published online on: October 15, 2014     https://doi.org/10.3892/mmr.2014.2649
  • Pages: 113-120

  • Copyright: © Hua et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Superparamagnetic iron oxide (SPIO) nanoparticles generate superparamagnetism, thereby resulting in an inhomogeneous local magnetic field, which shortens the T2 value on magnetic resonance imaging (MRI). The purpose of the present study was to use MRI to track bone marrow mesenchymal stem cells (BMSCs) labeled with SPIO in a rat model of myocardial infarction. The BMSCs were isolated from rats and labeled with SPIO. The anterior descending branch of the coronary artery was ligated under anesthesia. Two weeks later, the rats received, at random, 5x107 SPIO‑labeled BMSCs, 5x107 unlabeled BMSCs or a vehicle (100 µl phosphate‑buffered saline) via direct injection into the ischemic area (20 animals/group). MRI was used to track the SPIO‑labeled BMSCs and the rats were then sacrificed to verify the presence of BMSCs using immunohistochemistry with an anti‑CD90 antibody. The procedure labeled 99% of the BMSCs with SPIO, which exhibited low‑intensity signals on T2 and T2* MRI imaging. At 24 h post‑BMSC transplantation, low‑intensity MRI signals were detected on the T2 and T2* sequences at the infarction margins. After 3 weeks following transplantation, low‑intensity signals started to appear within the infarcted area; however, the signal intensity subsequently decreased and became indistinct. Immunohistochemistry revealed that the SPIO‑labeled BMSCs migrated from the margin into the infarcted region. In conclusion, the BMSCs were readily labeled with SPIO and in vivo and MRI tracking demonstrated that the SPIO‑labeled BMSCs established and grew in the infarcted myocardium.
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January-2015
Volume 11 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
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Spandidos Publications style
Hua P, Wang YY, Liu LB, Liu JL, Liu JY, Yang YQ and Yang SR: In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction. Mol Med Rep 11: 113-120, 2015
APA
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., & Yang, S. (2015). In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction. Molecular Medicine Reports, 11, 113-120. https://doi.org/10.3892/mmr.2014.2649
MLA
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., Yang, S."In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction". Molecular Medicine Reports 11.1 (2015): 113-120.
Chicago
Hua, P., Wang, Y., Liu, L., Liu, J., Liu, J., Yang, Y., Yang, S."In vivo magnetic resonance imaging tracking of transplanted superparamagnetic iron oxide‑labeled bone marrow mesenchymal stem cells in rats with myocardial infarction". Molecular Medicine Reports 11, no. 1 (2015): 113-120. https://doi.org/10.3892/mmr.2014.2649