Function of interleukin‑17 and ‑35 in the blood of patients with hepatitis B‑related liver cirrhosis

Shi,Min; Wei,Jue; Dong,Jinbin; Meng,Wenying; Ma,Jiali; Wang,Ting; Wang,Na; Wang,Yugang

doi:10.3892/mmr.2014.2681

Function of interleukin‑17 and ‑35 in the blood of patients with hepatitis B‑related liver cirrhosis

Authors:
- Min Shi
- Jue Wei
- Jinbin Dong
- Wenying Meng
- Jiali Ma
- Ting Wang
- Na Wang
- Yugang Wang
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Affiliations: Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, P.R. China
Published online on: October 16, 2014 https://doi.org/10.3892/mmr.2014.2681
Pages: 121-126
Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Intrahepatic T helper (Th)17 cytokine and serum interleukin (IL)‑17 levels in patients with hepatitis B are positively correlated with the progression of liver cirrhosis (LC). IL‑35 can significantly inhibit the differentiation of Th17 cells and the synthesis of IL‑17. The present study aimed to investigate the function and expression of IL‑17 and IL‑35 in the blood of patients with hepatitis B‑related LC. The levels of IL‑17 and IL‑35 in the peripheral blood of 30 patients with chronic hepatitis B (CHB), 79 with LC, 14 with chronic severe hepatitis B (CSHB), and 20 normal controls were detected by ELISA. Quantitative polymerase chain reaction was used to evaluate Epstein‑Barr virus‑induced gene 3 (EBI3), forkhead box (FOX)P3 and IL‑17 mRNA expression levels in peripheral blood mononuclear cells (PBMCs). Western blotting was used to determine protein expression. The liver function of patients and normal controls was measured. EBI3, IL‑17 and FOXP3 mRNA expression levels in PBMCs from patients with LC, CHB and CSHB were higher than those in cells from the controls. IL‑17 mRNA levels differed significantly according to the Child‑Pugh classification and exhibited an upward trend over time in contrast to a downward trend for EBI3 and FOXP3 mRNA. The changes in protein expression in the peripheral blood were consistent with the changes in mRNA expression. Serum IL‑17 levels were positively correlated with total bilirubin (TBIL), alanine aminotransferase (ALT) and Child‑Pugh grade, and were negatively correlated with albumin. These observed differences were significant. Serum IL‑35 levels were negatively correlated with albumin, but not with Child‑Pugh grade, ALT and TBIL. IL‑17 and IL‑35 may be critically involved in the pathogenesis of hepatitis B‑related LC.

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