Crude extract of Polygonum cuspidatum stimulates immune responses in normal mice by increasing the percentage of Mac‑3‑positive cells and enhancing macrophage phagocytic activity and natural killer cell cytotoxicity

Chueh,Fu‑Shin; Lin,Jen‑Jyh; Lin,Ju‑Hwa; Weng,Shu‑Wen; Huang,Yi‑Ping; Chung,Jing‑Gung

doi:10.3892/mmr.2014.2739

Crude extract of Polygonum cuspidatum stimulates immune responses in normal mice by increasing the percentage of Mac‑3‑positive cells and enhancing macrophage phagocytic activity and natural killer cell cytotoxicity

Authors:
- Fu‑Shin Chueh
- Jen‑Jyh Lin
- Ju‑Hwa Lin
- Shu‑Wen Weng
- Yi‑Ping Huang
- Jing‑Gung Chung
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Affiliations: Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, R.O.C. , School of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C., Department of Physiology, China Medical University, Taichung, Taiwan, R.O.C.
Published online on: October 22, 2014 https://doi.org/10.3892/mmr.2014.2739
Pages: 127-132
Copyright: © Chueh et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Polygonum cuspidatum is a natural plant that is used in traditional Chinese herbal medicine. The crude extract of Polygonum cuspidatum (CEPC) has numerous biological effects; however, there is a lack of studies on the effects of CEPC on immune responses in normal mice. The aim of the present study was to determine the in vivo effects of CEPC on immune responses in normal mice. CEPC (0, 50, 100, 150 and 200 mg/kg) was orally administered to BALB/c mice for three weeks, following which blood, liver, and spleen samples were collected. CEPC did not significantly affect the total body weight, or tissue weights of the liver or spleen, as compared with the control mice. CEPC increased the percentages of CD3 (T‑cell marker), 11b (monocytes) and Mac‑3 (macrophages) positive‑cells, and reduced the percentage of CD19‑positive cells (B‑cell marker), as compared with the control mice. CEPC (100 mg/kg) stimulated macrophage phagocytosis of blood samples but did not affect macrophage phagocytosis in the peritoneum. Activity of the splenic natural killer cells was increased in response to CEPC (50 mg/kg) treatment. Furthermore, CEPC inhibited T‑ and B‑cell proliferation when the cells were stimulated with concanavalin A and lipopolysaccharide, respectively.

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