Inhibition of SATB1 expression in regulatory T cells contributes to hepatitis B virus-related chronic liver inflammation

  • Authors:
    • Xue-Fen Li
    • Li Tian
    • Yi-Yin Wang
    • Hai-Shen Kong
    • Yue-Jiao Dong
    • Qiao-Yun Zhu
    • Qin Ni
    • Yu Chen
  • View Affiliations

  • Published online on: October 23, 2014     https://doi.org/10.3892/mmr.2014.2752
  • Pages: 231-236
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Abstract

Regulatory T cells (Tregs) contribute to the pathogenesis of chronic hepatitis B (CHB). Special AT-rich sequence-binding protein 1 (SATB1) may be a key component of this process. In the present study, Tregs and conventional T cells (Tconvs) were isolated by magnetic cell sorting of peripheral blood from CHB patients (n=57), individuals with resolved hepatitis B virus (HBV) infections (n=15), and healthy controls (n=29). SATB1 expression was studied by reverse transcription‑quantitative PCR, flow cytometry and immunofluorescence microscopy, and the correlation of SATB1 expression to the expression of liver inflammation serum markers and the HBV DNA load was assessed. CHB patients showed significantly reduced SATB1 expression in Tregs than healthy controls and individuals with resolved HBV infections. Moreover, SATB1 expression in Tregs was significantly lower than in Tconvs of patients with chronic HBV infection. Serum HBV DNA and liver inflammation markers were inversely correlated to the SATB1 mRNA level in Tregs. Antiviral treatment was accompanied by increased expression of the SATB1 gene in Tregs. Thus, Tregs from CHB patients have reduced levels of SATB1, which is resolved with antiviral therapy. Inhibition of SATB1 expression may impair the hepatic inflammatory response and contribute to HBV persistence.
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January-2015
Volume 11 Issue 1

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Spandidos Publications style
Li X, Tian L, Wang Y, Kong H, Dong Y, Zhu Q, Ni Q and Chen Y: Inhibition of SATB1 expression in regulatory T cells contributes to hepatitis B virus-related chronic liver inflammation. Mol Med Rep 11: 231-236, 2015.
APA
Li, X., Tian, L., Wang, Y., Kong, H., Dong, Y., Zhu, Q. ... Chen, Y. (2015). Inhibition of SATB1 expression in regulatory T cells contributes to hepatitis B virus-related chronic liver inflammation. Molecular Medicine Reports, 11, 231-236. https://doi.org/10.3892/mmr.2014.2752
MLA
Li, X., Tian, L., Wang, Y., Kong, H., Dong, Y., Zhu, Q., Ni, Q., Chen, Y."Inhibition of SATB1 expression in regulatory T cells contributes to hepatitis B virus-related chronic liver inflammation". Molecular Medicine Reports 11.1 (2015): 231-236.
Chicago
Li, X., Tian, L., Wang, Y., Kong, H., Dong, Y., Zhu, Q., Ni, Q., Chen, Y."Inhibition of SATB1 expression in regulatory T cells contributes to hepatitis B virus-related chronic liver inflammation". Molecular Medicine Reports 11, no. 1 (2015): 231-236. https://doi.org/10.3892/mmr.2014.2752