Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice

Cho,Soo‑Min; Park,Jin‑A; Kim,Na‑Hyun; Kim,Dong‑Soon; Zhang,Dan; Yi,Hee; Cho,Hee‑Jung; Kim,Ja  Kyung; Lee,Dong  Ki; Kim,Jin‑Suk; Shin,Ho‑Chul

doi:10.3892/mmr.2014.2687

Effect of eicosapentaenoic acid on cholesterol gallstone formation in C57BL/6J mice

Authors:
- Soo‑Min Cho
- Jin‑A Park
- Na‑Hyun Kim
- Dong‑Soon Kim
- Dan Zhang
- Hee Yi
- Hee‑Jung Cho
- Ja Kyung Kim
- Dong Ki Lee
- Jin‑Suk Kim
- Ho‑Chul Shin
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Affiliations: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143‑701, Republic of Korea, Department of Internal Medicine, Yonsei University College of Medicine, Seoul 120‑752, Republic of Korea
Published online on: October 17, 2014 https://doi.org/10.3892/mmr.2014.2687
Pages: 362-366

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Abstract

The present study investigated the preventive effect of ω‑3 fatty acids against cholesterol gallstone (CG) formation. CG formation was induced in C57BL/6J mice using a lithogenic diet (LD). The mice were divided into four treatment groups: i) LD, ii) LD plus eicosapentaenoic acid (EPA), iii) LD plus docosahexaenoic acid (DHA) and iv) LD plus EPA plus DHA. Subsequent to feeding the mice the LD for four weeks, EPA and/or DHA (70 mg/kg/day) were orally administered for eight weeks. The mice in the EPA treatment groups exhibited significantly less gallstone formation than those in the LD group. By contrast, DHA treatment only slightly suppressed gallstone formation. The expression of mucin 2, 5AC, 5B and 6 was significantly decreased in the gallbladders of mice in the EPA groups (70‑90%) and the LD plus DHA group (30‑50%), compared with that in the mice in the LD group. In addition, the mRNA expression of 3‑hydroxy‑3‑methylglutaryl‑coenzyme A reductase was significantly decreased in the livers of mice in the EPA treatment group compared with that in the livers of mice in the LD group. In conclusion, EPA was found to have a dominant anti‑lithogenic effect in C57BL/6J mice.

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