MicroRNA‑106a confers cisplatin resistance in non‑small cell lung cancer A549 cells by targeting adenosine triphosphatase‑binding cassette A1

Ma,Yanxin; Li,Xuenan; Cheng,Song; Wei,Wei; Li,Yaming

doi:10.3892/mmr.2014.2688

MicroRNA‑106a confers cisplatin resistance in non‑small cell lung cancer A549 cells by targeting adenosine triphosphatase‑binding cassette A1

Authors:
- Yanxin Ma
- Xuenan Li
- Song Cheng
- Wei Wei
- Yaming Li
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Affiliations: Department of Nuclear Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Nuclear Medicine, General Hospital of Daqing Oil Field, Daqing, Heilongjiang 163000, P.R. China
Published online on: October 17, 2014 https://doi.org/10.3892/mmr.2014.2688
Pages: 625-632

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Abstract

MicroRNAs (miRNAs) have been discovered to have pivotal roles in regulating the drug resistance of various types of human cancer, including cisplatin (DDP) resistance in non‑small cell lung cancer (NSCLC). Fewer studies have explored the roles of miR‑106a in NSCLC-cell resistance to DDP and its precise molecular mechanism has remained elusive. In the present study, whether miR‑106a was able to mediate resistance of the lung cancer cell line A549 to DDP was investigated. Reverse transcription quantitative polymerase chain reaction was used to analyze miR-106a mRNA expression levels. miR‑106a expression levels were upregulated in the DDP-resistant cell line A549/DDP compared with its parental cell line, A549. miR‑106a-transfection induced DDP‑resistance in A549 cells, while repression of miR‑106a by anti‑miR‑106a in A549/DDP resulted in enhanced DDP cytotoxicity. Furthermore, it was discovered that the mechanism of miR‑106a‑induced DDP resistance involved the expression of adenosine triphosphatase‑binding cassette, sub‑family A, member 1 (ABCA1), as indicated by transfection of cells with short interfering RNA-ABCA1. The results of the present study suggested a novel mechanism underlying DDP-resistance in NSCLC.

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