Inhibitory effects of oligochitosan on TNF‑α, IL‑1β and nitric oxide production in lipopolysaccharide‑induced RAW264.7 cells

Zhu,Jie; Zhang,Yuanyuan; Wu,Guoyou; Xiao,Zhen; Zhou,Huanqin; Yu,Xiaomei

doi:10.3892/mmr.2014.2643

Inhibitory effects of oligochitosan on TNF‑α, IL‑1β and nitric oxide production in lipopolysaccharide‑induced RAW264.7 cells

Authors:
- Jie Zhu
- Yuanyuan Zhang
- Guoyou Wu
- Zhen Xiao
- Huanqin Zhou
- Xiaomei Yu
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Affiliations: Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Respiratory Medicine, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China
Published online on: October 15, 2014 https://doi.org/10.3892/mmr.2014.2643
Pages: 729-733

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Abstract

Oligochitosan has been reported to possess anti‑inflammatory properties; however, the mechanism of the anti‑inflammatory effects of oligochitosan remains unknown. The present study aimed to investigate the expression levels of inflammatory cytokines and the production of nitric oxide (NO), in the nuclear factor (NF)‑κB pathway of lipopolysaccharide (LPS)‑stimulated RAW264.7 murine macrophages. The results of the present study demonstrated that different concentrations of oligochitosan could significantly lower the levels of NO, tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β, released from LPS‑stimulated RAW264.7 cells. This was shown to be mediated through inhibiting the activation of the NF‑κB pathway. These results demonstrate that oligochitosan may efficiently inhibit inflammation and has the potential to be an effective anti‑inflammatory agent.

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