GABAA receptor overexpression in the lateral hypothalamic area attenuates gastric ischemia‑reperfusion injury in rats

Gao,Lin; Zhu,Tao; Xie,Guilin; Lou,Xiangxin; Li,Shibao; Zhou,Yan; Deng,Zhenxu; Chu,Dechang; Lou,Jiyu; Du,Dongshu

doi:10.3892/mmr.2014.2816

GABAA receptor overexpression in the lateral hypothalamic area attenuates gastric ischemia‑reperfusion injury in rats

Authors:
- Lin Gao
- Tao Zhu
- Guilin Xie
- Xiangxin Lou
- Shibao Li
- Yan Zhou
- Zhenxu Deng
- Dechang Chu
- Jiyu Lou
- Dongshu Du
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Affiliations: Department of Neurology, The Affiliated Second Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Shanghai 200444, P.R. China, Life Science College of Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China, Department of Bioengineering, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P.R. China, Department of Laboratory Medicine, Lianyungang Hospital Affiliated Bengbu Medical College, Lianyungang, Jiangsu 222006, P.R. China, Department of Life Science, Heze University, Heze, Shandong 274500, P.R. China
Published online on: October 29, 2014 https://doi.org/10.3892/mmr.2014.2816
Pages: 1057-1062

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Abstract

Excessive activation of the greater splanchnic nerve (GSN) has previously been determined to contribute to the progression of gastric ischemia‑reperfusion (GI‑R) injury. The present study was designed to estimate the protective effects of GABAA receptor (GABAAR) overexpression in the lateral hypothalamic area (LHA) against GI‑R injury. The GI‑R injury model was induced in rats by clamping the celiac artery for 30 min and then reperfusing for 1 h. Microinjection of recombinant adenoviral vectors overexpressing GABAAR (Ad‑GABAAR) or control adenoviral vectors (Ad‑Con) into the LHA was conducted in GI‑R and normal control rats. Significant protective effects were observed on day 2 after Ad‑GABAAR treatment in the GI‑R injury rats. Ad‑GABAAR treatment reduced plasma norepinephrine levels, plasma angiotensin II levels and peripheral GSN activity, but increased the gastric mucosal blood flow, as compared with Ad‑Con treatment. These results indicate that adenoviral vector‑induced GABAAR overexpression in the LHA blunts GSN activity and subsequently alleviates the effects of gastric injury in GI‑R rats.

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