Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells

  • Authors:
    • Zhe Chen
    • Hongfei Jin
    • Yahui Hou
    • Bo Wang
    • Chunlin Chen
    • Ziyi Yang
    • Dongbing Ding
    • Yunfei Zuo
    • Shuangyi Ren
  • View Affiliations

  • Published online on: October 27, 2014     https://doi.org/10.3892/mmr.2014.2776
  • Pages: 1542-1546
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aims of the current study were to systematically analyze the regulation of the expression of NALP3 by the P2X7 receptor in P388D1 murine macrophage‑like cells, and to investigate the association between the P2X7 receptor and the NALP3 at the molecular level. Cell culture, RNA transfection, adenosine triphosphate (ATP)‑induced expression of NALP3, reverse transcription polymerase chain reaction and western blotting were used to explore the association between the P2X7 receptor and NALP3‑encoding gene in P388D1 murine macrophage‑like cells at the molecular level. The ATP‑activated P2X7 receptor can induce the upregulation of NALP3 expression at the gene and protein levels in P388D1 cells. These results demonstrated that the activation of P2X7 increases the expression levels of NALP3 in P388D1 murine macrophage‑like cells.
View Figures
View References

Related Articles

Journal Cover

February-2015
Volume 11 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen Z, Jin H, Hou Y, Wang B, Chen C, Yang Z, Ding D, Zuo Y and Ren S: Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells. Mol Med Rep 11: 1542-1546, 2015
APA
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z. ... Ren, S. (2015). Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells. Molecular Medicine Reports, 11, 1542-1546. https://doi.org/10.3892/mmr.2014.2776
MLA
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z., Ding, D., Zuo, Y., Ren, S."Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells". Molecular Medicine Reports 11.2 (2015): 1542-1546.
Chicago
Chen, Z., Jin, H., Hou, Y., Wang, B., Chen, C., Yang, Z., Ding, D., Zuo, Y., Ren, S."Activated P2X7 receptor upregulates the expression levels of NALP3 in P388D1 murine macrophage‑like cells". Molecular Medicine Reports 11, no. 2 (2015): 1542-1546. https://doi.org/10.3892/mmr.2014.2776