Open Access

Ephrin type‑A receptor 2 regulates sensitivity to paclitaxel in nasopharyngeal carcinoma via the phosphoinositide 3‑kinase/Akt signalling pathway

  • Authors:
    • Yunyun Wang
    • Yong Liu
    • Guo Li
    • Zhongwu Su
    • Shuling Ren
    • Pingqing  Tan
    • Xin Zhang
    • Yuanzheng Qiu
    • Yongquan Tian
  • View Affiliations

  • Published online on: October 29, 2014     https://doi.org/10.3892/mmr.2014.2799
  • Pages: 924-930
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Ephrin type‑A receptor 2 (EphA2) is a receptor tyrosine kinase that is associated with cancer cell metastasis. There has been little investigation into its impact on the regulation of sensitivity to paclitaxel in nasopharyngeal carcinoma (NPC). In the present study, upregulation of EphA2 expression enhanced the survival of NPC 5‑8F cells, compared with control cells exposed to the same concentrations of paclitaxel. Flow cytometry and western blot analysis demonstrated that over‑expression of EphA2 decreased NPC cancer cell sensitivity to paclitaxel by regulating paclitaxel‑mediated cell cycle progression but not apoptosis in vitro. This was accompanied by alterations in the expression of cyclin‑dependent kinase inhibitors, p21 and p27, and of inactive phosphorylated‑retinoblastoma protein. Furthermore, paclitaxel stimulation and EphA2 over‑expression resulted in activation of the phosphoinositide 3‑kinase (PI3K)/Akt signalling pathway in NPC cells. Inhibition of the PI3K/Akt signalling pathway restored sensitivity to paclitaxel in 5‑8F cells over‑expressing EphA2, which indicated that the PI3K/Akt pathway is involved in EphA2‑mediated paclitaxel sensitivity. The current study demonstrated that EphA2 mediates sensitivity to paclitaxel via the regulation of the PI3K/Akt signalling pathway in NPC.
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February-2015
Volume 11 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang Y, Liu Y, Li G, Su Z, Ren S, Tan P, Zhang X, Qiu Y and Tian Y: Ephrin type‑A receptor 2 regulates sensitivity to paclitaxel in nasopharyngeal carcinoma via the phosphoinositide 3‑kinase/Akt signalling pathway. Mol Med Rep 11: 924-930, 2015
APA
Wang, Y., Liu, Y., Li, G., Su, Z., Ren, S., Tan, P. ... Tian, Y. (2015). Ephrin type‑A receptor 2 regulates sensitivity to paclitaxel in nasopharyngeal carcinoma via the phosphoinositide 3‑kinase/Akt signalling pathway. Molecular Medicine Reports, 11, 924-930. https://doi.org/10.3892/mmr.2014.2799
MLA
Wang, Y., Liu, Y., Li, G., Su, Z., Ren, S., Tan, P., Zhang, X., Qiu, Y., Tian, Y."Ephrin type‑A receptor 2 regulates sensitivity to paclitaxel in nasopharyngeal carcinoma via the phosphoinositide 3‑kinase/Akt signalling pathway". Molecular Medicine Reports 11.2 (2015): 924-930.
Chicago
Wang, Y., Liu, Y., Li, G., Su, Z., Ren, S., Tan, P., Zhang, X., Qiu, Y., Tian, Y."Ephrin type‑A receptor 2 regulates sensitivity to paclitaxel in nasopharyngeal carcinoma via the phosphoinositide 3‑kinase/Akt signalling pathway". Molecular Medicine Reports 11, no. 2 (2015): 924-930. https://doi.org/10.3892/mmr.2014.2799