Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells

Deng,Ying‑Hu; Li,Li‑Hua; He,Jin; Li,Mei; Zhang,Yu; Wang,Xiu‑Mei; Cui,Fu‑Zhai; Xia,Hong

doi:10.3892/mmr.2014.2876

Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells

Authors:
- Ying‑Hu Deng
- Li‑Hua Li
- Jin He
- Mei Li
- Yu Zhang
- Xiu‑Mei Wang
- Fu‑Zhai Cui
- Hong Xia
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Affiliations: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Spinal Surgery, Hospital of Orthopedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China, Department of Materials Science and Engineering, Institute of Regenerative and Biomimetic Materials, Tsinghua University, Beijing 100084, P.R. China
Published online on: November 6, 2014 https://doi.org/10.3892/mmr.2014.2876
Pages: 975-981
Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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