MicroRNA-451a is associated with cell proliferation, migration and apoptosis in renal cell carcinoma

Su,Zhengming; Ni,Liangchao; Yu,Wenshui; Yu,Zuhu; Chen,Duqun; Zhang,Enpu; Li,Yifan; Wang,Yadong; Li,Xianxin; Yang,Shangqi; Gui,Yaoting; Lai,Yongqing; Ye,Jiongxian

doi:10.3892/mmr.2014.2957

MicroRNA-451a is associated with cell proliferation, migration and apoptosis in renal cell carcinoma

Authors:
- Zhengming Su
- Liangchao Ni
- Wenshui Yu
- Zuhu Yu
- Duqun Chen
- Enpu Zhang
- Yifan Li
- Yadong Wang
- Xianxin Li
- Shangqi Yang
- Yaoting Gui
- Yongqing Lai
- Jiongxian Ye
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Affiliations: Department of Urology, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China, Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Department of Urology, Zunyi Medical College Fifth Affiliated Hospital, Zhuhai, Guangdong 519100, P.R. China
Published online on: November 14, 2014 https://doi.org/10.3892/mmr.2014.2957
Pages: 2248-2254

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Abstract

MicroRNAs (miRNAs) are an important class of small, non‑coding RNA molecules that regulate gene expression at the transcriptional or post‑transcriptional level. They are involved in apoptosis, proliferation and migration and are known to have an important role in many types of cancer. Aberrant expression of miRNA‑451a (miR‑451a) has previously been reported in tumors, however its role in renal cell carcinoma (RCC) is currently unknown. The aim of the present study was to investigate the role of miR‑451a in RCC. The expression of miR‑451a was analyzed in 50 paired RCC and normal tissues by quantitative polymerase chain reaction. Furthermore, the effects of miR‑451a on cell migration, proliferation and apoptosis were evaluated, using migration scratch, MTT and flow cytometric assays. The present study demonstrated that miR‑451a was upregulated in RCC, as compared with paired normal tissues (P<0.05). Downregulation of miR‑451a using a synthesized inhibitor, significantly suppressed cell migration and proliferation, and induced apoptosis of renal cancer cells in vitro, as compared with a negative control (P<0.05). In the present study, it was determined that miR‑451a may have an important role as a tumor enhancer in RCC. These results imply that miR‑451a may be a promising therapeutic target for the treatment of RCC.

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1	Siegel R, Naishadham D and Jemal A: Cancer statistics, 2012. CA Cancer J Clin. 62:10–29. 2012. View Article : Google Scholar : PubMed/NCBI
2	Leroy X, Zini L, Buob D, Ballereau C, Villers A and Aubert S: Renal cell carcinoma with rhabdoid features: an aggressive neoplasm with overexpression of p53. Arch Pathol Lab Med. 131:102–106. 2007.PubMed/NCBI
3	Zhao P, Dai M, Chen W and Li N: Cancer trends in China. Jpn J Clin Oncol. 40:281–285. 2010. View Article : Google Scholar : PubMed/NCBI
4	Patel C, Ahmed A and Ellsworth P: Renal cell carcinoma: a reappraisal. Urol Nurs. 32:182–190. 2012.PubMed/NCBI
5	Wei C, Wu S, Li X, et al: High expression of FER tyrosine kinase predicts poor prognosis in clear cell renal cell carcinoma. Oncol Lett. 5:473–478. 2013.PubMed/NCBI
6	Yu XY, Zhang Z, Liu J, Zhan B and Kong CZ: MicroRNA-141 is downregulated in human renal cell carcinoma and regulates cell survival by targeting CDC25B. Onco Targets Ther. 6:349–354. 2013.PubMed/NCBI
7	Zhai Q, Zhou L, Zhao C, et al: Identification of miR-508-3p and miR-509-3p that are associated with cell invasion and migration and involved in the apoptosis of renal cell carcinoma. Biochem Biophys Res Commun. 419:621–626. 2012. View Article : Google Scholar : PubMed/NCBI
8	Yu Z, Ni L, Chen D, et al: Identification of miR-7 as an oncogene in renal cell carcinoma. J Mol Histol. 44:669–677. 2013. View Article : Google Scholar : PubMed/NCBI
9	Yang XW, Zhang LJ, Huang XH, et al: miR-145 suppresses cell invasion in hepatocellular carcinoma cells: miR-145 targets ADAM17. Hepatol Res. 4:551–559. 2014. View Article : Google Scholar
10	Xi Y: MicroRNA: A New Player for Cancer Chemoprevention. J Integr Oncol. 2:2013. View Article : Google Scholar : PubMed/NCBI
11	Xi JJ: MicroRNAs in Cancer. Cancer Treat Res. 158:119–137. 2013. View Article : Google Scholar : PubMed/NCBI
12	Bian HB, Pan X, Yang JS, Wang ZX and De W: Upregulation of microRNA-451 increases cisplatin sensitivity of non-small cell lung cancer cell line (A549). J Exp Clin Cancer Res. 30:202011. View Article : Google Scholar : PubMed/NCBI
13	Bergamaschi A and Katzenellenbogen BS: Tamoxifen downregulation of miR-451 increases 14-3-3ζ and promotes breast cancer cell survival and endocrine resistance. Oncogene. 31:39–47. 2012. View Article : Google Scholar
14	Rani SB, Rathod SS, Karthik S, Kaur N, Muzumdar D and Shiras AS: MiR-145 functions as a tumor-suppressive RNA by targeting Sox9 and adducin 3 in human glioma cells. Neuro Oncol. 15:1302–1316. 2013. View Article : Google Scholar : PubMed/NCBI
15	Yi Z, Fu Y, Zhao S, Zhang X and Ma C: Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues. J Cancer Res Clin Oncol. 136:855–862. 2010. View Article : Google Scholar
16	Yoshino H, Enokida H, Itesako T, et al: Tumor-suppressive microRNA-143/145 cluster targets hexokinase-2 in renal cell carcinoma. Cancer Sci. 104:1567–1574. 2013. View Article : Google Scholar : PubMed/NCBI
17	Zhou L, Chen J, Li Z, et al: Integrated profiling of microRNAs and mRNAs: microRNAs located on Xq27.3 associate with clear cell renal cell carcinoma. PloS One. 5:e152242010. View Article : Google Scholar
18	Zhang J, Yang Y, Yang T, et al: microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity. Br J Cancer. 103:1215–1220. 2010. View Article : Google Scholar : PubMed/NCBI
19	Kefas B, Godlewski J, Comeau L, et al: microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma. Cancer Res. 68:3566–3572. 2008. View Article : Google Scholar : PubMed/NCBI
20	Sun H, Li QW, Lv XY, et al: MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation. Mol Biol Rep. 37:2951–2958. 2010. View Article : Google Scholar
21	Yamamura S, Saini S, Majid S, et al: MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma. Carcinogenesis. 33:294–300. 2012. View Article : Google Scholar :
22	Redova M, Poprach A, Besse A, et al: MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma. Tumour Biol. 34:481–491. 2013. View Article : Google Scholar
23	Zhang H, Guo Y, Shang C, Song Y and Wu B: miR-21 downregulated TCF21 to inhibit KISS1 in renal cancer. Urology. 80:1298–1302. 2012. View Article : Google Scholar : PubMed/NCBI
24	Nakada C, Matsuura K, Tsukamoto Y, et al: Genome-wide microRNA expression profiling in renal cell carcinoma: significant down-regulation of miR-141 and miR-200c. J Pathol. 216:418–427. 2008. View Article : Google Scholar : PubMed/NCBI
25	Boguslawska J, Wojcicka A, Piekielko-Witkowska A, Master A and Nauman A: MiR-224 targets the 3′UTR of type 1 5′-iodothyronine deiodinase possibly contributing to tissue hypothyroidism in renal cancer. PloS One. 6:e245412011. View Article : Google Scholar
26	Wang R, Wang ZX, Yang JS, Pan X, De W and Chen LB: MicroRNA-451 functions as a tumor suppressor in human non-small cell lung cancer by targeting ras-related protein 14 (RAB14). Oncogene. 30:2644–2658. 2011. View Article : Google Scholar : PubMed/NCBI
27	Bandres E, Bitarte N, Arias F, et al: microRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells. Clin Cancer Res. 15:2281–2290. 2009. View Article : Google Scholar : PubMed/NCBI
28	Godlewski J, Nowicki MO, Bronisz A, et al: MicroRNA-451 regulates LKB1/AMPK signaling and allows adaptation to metabolic stress in glioma cells. Mol Cell. 37:620–632. 2010. View Article : Google Scholar : PubMed/NCBI
29	Kim VN: MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol. 6:376–385. 2005. View Article : Google Scholar : PubMed/NCBI
30	Hammond SM: RNAi, microRNAs, and human disease. Cancer Chemother Pharmacol. 58(Suppl 1): s63–s68. 2006. View Article : Google Scholar : PubMed/NCBI
31	Drakaki A and Iliopoulos D: MicroRNA gene networks in oncogenesis. Curr Genomics. 10:35–41. 2009. View Article : Google Scholar : PubMed/NCBI