Endoplasmic reticulum stress protects human thyroid carcinoma cell lines against ionizing radiation-induced apoptosis

  • Authors:
    • Xin‑Yu Wu
    • Rui‑Tai Fan
    • Xin‑Hui Yan
    • Jing Cui
    • Jun‑Ling Xu
    • Hao Gu
    • Yong‑Ju Gao
  • View Affiliations

  • Published online on: November 14, 2014     https://doi.org/10.3892/mmr.2014.2956
  • Pages: 2341-2347
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Abstract

Radiotherapy is one of the most effective forms of cancer treatment, used in the treatment of a number of malignant tumors. However, the resistance of tumor cells to ionizing radiation remains a major therapeutic problem and the critical mechanisms determining radiation resistance are poorly defined. In the present study, a cellular endoplasmic reticulum (ER) stress microenvironment was established through the pretreatment of cultured thyroid cancer cells with tunicamycin (TM) and thapsigargin (TG), in order to mimic the ER stress response in a tumor microenvironment. This microenviroment was confirmed through the X‑box binding protein 1 splice process, glucose‑regulated protein 78 kD and ER degradation‑enhancing α‑mannosidase‑like mRNA expression. A clonogenic assay was used to measure cancer cell resistance to 60Co‑γ following TM pretreatment; in addition, human C/EBP homologous protein (CHOP) mRNA expression was determined and apoptosis assays were performed. The results showed that TM or TG pretreatment inhibited CHOP expression and reduced the apoptotic rate of cells. Furthermore, the results demonstrated that the induced ER stress response rendered cancer cells more resistant to ionizing radiation‑induced apoptosis. Therefore, the ER stress pathway may be a potential therapeutic target in order to improve the clinical efficiency of radiotherapy.

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March-2015
Volume 11 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Wu XY, Fan RT, Yan XH, Cui J, Xu JL, Gu H and Gao YJ: Endoplasmic reticulum stress protects human thyroid carcinoma cell lines against ionizing radiation-induced apoptosis. Mol Med Rep 11: 2341-2347, 2015.
APA
Wu, X., Fan, R., Yan, X., Cui, J., Xu, J., Gu, H., & Gao, Y. (2015). Endoplasmic reticulum stress protects human thyroid carcinoma cell lines against ionizing radiation-induced apoptosis. Molecular Medicine Reports, 11, 2341-2347. https://doi.org/10.3892/mmr.2014.2956
MLA
Wu, X., Fan, R., Yan, X., Cui, J., Xu, J., Gu, H., Gao, Y."Endoplasmic reticulum stress protects human thyroid carcinoma cell lines against ionizing radiation-induced apoptosis". Molecular Medicine Reports 11.3 (2015): 2341-2347.
Chicago
Wu, X., Fan, R., Yan, X., Cui, J., Xu, J., Gu, H., Gao, Y."Endoplasmic reticulum stress protects human thyroid carcinoma cell lines against ionizing radiation-induced apoptosis". Molecular Medicine Reports 11, no. 3 (2015): 2341-2347. https://doi.org/10.3892/mmr.2014.2956