Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329

Meng,Du; Liu,Rui; Pei,Li; Hou,Lei; Ning,Qian; Yu,Qing; Feng,Lu; Zhao,Xinhan

doi:10.3892/mmr.2014.3043

Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells

Corrigendum in: /mmr/12/6/8329

Authors:
- Du Meng
- Rui Liu
- Li Pei
- Lei Hou
- Qian Ning
- Qing Yu
- Lu Feng
- Xinhan Zhao
View Affiliations

Affiliations: Department of Oncology, First Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: December 3, 2014 https://doi.org/10.3892/mmr.2014.3043
Pages: 2555-2561
Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to investigate the feasibility of lentiviral‑mediated Cys‑Asn‑Gly‑Arg‑Cys (CNGRC) peptide gene transduction in adipose stem cells. Adipose stem cells were prepared using enzymatic digestion and repeated adherence methods and identified in culture by immunofluorescence staining of surface markers. The pluripotency of the cultured adipose stem cells was confirmed by their induced differentiation into bone and fat cells. Following polymerase chain reaction amplification, the gene sequence for the CNGRC peptide was cloned into a lentiviral vector, which was then co‑transfected into 293T cells with packaging plasmids Helper 1.0 and Helper 2.0. The lentiviruses carrying the CNGRC peptide gene were then harvested and used to transfect adipose stem cells. Following transduction, expression of CNGRC in adipose stem cells was detected using western blot analysis. Adipose stem cells in culture were successfully induced to differentiate into adipocytes and osteoblasts and the lentiviral vector containing CNGRC‑3Flag‑EGFP was successfully constructed. Following transduction, western blot analysis and immunofluorescence staining demonstrated expression of the CNGRC protein in adipose stem cells. This suggested that adipose stem cell lines expressing the CNGRC peptide were successfully established.

View Figures

View References

1	Matsumoto Y, Iwasaki H and Suda T: Maintenance of adult stem cells: Role of the stem cell niche. Adult Stem Cells. Springer Science+Business Media, LLC; pp. 35–55. 2011, View Article : Google Scholar
2	Takeda N, Jain R, LeBoeuf MR, et al: Interconversion between intestinal stem cell populations in distinct niches. Science. 334:1420–1424. 2011. View Article : Google Scholar : PubMed/NCBI
3	Kuhbier JW, Weyand B, Radtke C, et al: Isolation, characterization, differentiation and application of adipose-derived stem cells. Adv Biochem Eng Biotechnol. 123:55–105. 2010.
4	Josiah DT, Zhu D, Dreher F, et al: Adipose-derived stem cells as therapeutic delivery vehicles of an oncolytic virus for glioblastoma. Mol Ther. 18:377–385. 2010. View Article : Google Scholar :
5	Schäffler A and Büchler C: Concise review: adipose tissue-derived stromal cells-Basic and clinical implications for novel cell-based therapies. Stem Cells. 25:818–827. 2007. View Article : Google Scholar
6	Cho JA, Park H, Lim EH, et al: Exosomes from ovarian cancer cells induce adipose tissue-derived mesenchymal stem cells to acquire the physical and functional characteristics of tumor-supporting myofibroblasts. Gynecol Oncol. 123:379–386. 2011. View Article : Google Scholar : PubMed/NCBI
7	Rowan BG, Gimble JM, Sheng M, et al: Human adipose tissue-derived stromal/stem cells promote migration and early metastasis of triple negative breast cancer xenografts. PLoS One. 28:e895952014. View Article : Google Scholar
8	Park YM, Yoo SH and Kim SH: Adipose-derived stem cells induced EMT-like changes in H358 lung cancer cells. Anticancer Res. 33:4421–4430. 2013.PubMed/NCBI
9	Hou L, Zhao X, Wang P, et al: Antitumor activity of antimicrobial peptides containing CisoDGRC in CD13 negative breast cancer cells. PLoS One. 8:e534912013. View Article : Google Scholar : PubMed/NCBI
10	Robak LA, Venkatesh K, Lee H, et al: Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition. J Neurosci. 29:5768–5783. 2009. View Article : Google Scholar : PubMed/NCBI
11	Curnis F, Cattaneo A, Longhi R, et al: Critical role of flanking residues in NGR-to-isoDGR transition and CD13/integrin receptor switching. J Biol Chem. 285:9114–9123. 2010. View Article : Google Scholar : PubMed/NCBI
12	Ndinguri MW, Solipuram R, Gambrell RP, et al: Peptide targeting of platinum anti-cancer drugs. Bioconjug Chem. 20:1869–1878. 2009. View Article : Google Scholar : PubMed/NCBI
13	Yokoyama Y and Ramakrishnan S: Addition of an aminopeptidase N-binding sequence to human endostatin improves inhibition of ovarian carcinoma growth. Cancer. 104:321–331. 2005. View Article : Google Scholar : PubMed/NCBI
14	Liu Y, Wei H, Zhen Y, et al: Relationship between receptor expression on cell surface of several solid tumors and tumor sensitivity to IFN-α 2a. Journal of the Fourth Military Medical University. 10:865–868. 2008.(In Chinese).
15	Meng J, Ma N, Yan Z, et al: NGR enhanced the anti-angiogenic activity of tum-5. J Biochem. 140:299–304. 2006. View Article : Google Scholar : PubMed/NCBI
16	Corti A, Pastorino F, Curnis F, et al: Targeted drug delivery and penetration into solid tumors. Med Res Rev. 32:1078–1091. 2012. View Article : Google Scholar
17	Curnis F, Sacchi A and Corti A: Improving chemotherapeutic drug penetration in tumors by vascular targeting and barrier alteration. J Clin Invest. 110:475–482. 2002. View Article : Google Scholar : PubMed/NCBI
18	Podolska K, Stachurska A, Hajdukiewicz K, et al: Gene therapy prospects - intranasal delivery of therapeutic genes. Adv Clin Exp Med. 21:525–534. 2012.PubMed/NCBI
19	Christ M, Lusky M, Stoeckel F, et al: Gene therapy with recombinant adenovirus vectors: evaluation of the host immune response. Immunol Lett. 57:19–25. 1997. View Article : Google Scholar : PubMed/NCBI
20	Zhou YF: In vitro study of HCN4 transfected biological pacemaker cells using lentivirus. Soochow University. 47–49. 2009.
21	Zhao L, Wu J, Zhou H, et al: Local gene delivery for cancer therapy. Curr Gene Ther. 11:423–432. 2011. View Article : Google Scholar : PubMed/NCBI
22	Liu YP and Berkhout B: miRNA cassettes in viral vectors: problems and solutions. Biochim Biophys Acta. 1809.732–745. 2011.
23	Nery AA, Nascimento IC, Glaser T, et al: Human mesenchymal stem cells: from immunophenotyping by flow cytometry to clinical applications. Cytometry A. 83:48–61. 2013. View Article : Google Scholar
24	Zuk PA, Zhu M, Ashjian PH, et al: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 13:4279–4295. 2002. View Article : Google Scholar : PubMed/NCBI
25	Rangappa S, Fen C, Lee EH, et al: Transformation of adult mesenchymal stem cells isolated from the fatty tissue into cardiomyocytes. The Annals of thoracic surgery. 75:775–779. 2003. View Article : Google Scholar : PubMed/NCBI
26	Zuk PA, Zhu M, Mizuno H, et al: Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI
27	Araña M, Mazo M, Aranda P, et al: Adipose tissue-derived mesenchymal stem cells: isolation, expansion and characterization. Methods Mol Biol. 1036:47–61. 2013. View Article : Google Scholar
28	Bacigalupo A, Socié G, Schrezenmeier H, et al: Bone marrow versus peripheral blood as the stem cell source for sibling transplants in acquired aplastic anemia: survival advantage for bone marrow in all age groups. Haematologica. 97:1142–1148. 2012. View Article : Google Scholar : PubMed/NCBI
29	Aust L, Devlin B, Foster SJ, et al: Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy. 6:7–14. 2004. View Article : Google Scholar : PubMed/NCBI
30	Dragoo JL, Choi JY, Lieberman JR, et al: Bone induction by BMP-2 transduced stem cells derived from human fat. J Orthop Res. 21:622–629. 2003. View Article : Google Scholar : PubMed/NCBI
31	Sun XZ, Liu GH, Wang ZQ, et al: Over-expression of VEGF165 in the adipose tissue-derived stem cells via the lentiviral vector. Chin Med J (Engl). 124:3093–3097. 2011.
32	Liu Y, Chen C, He H, et al: Lentiviral-mediated gene transfer into human adipose-derived stem cells: role of NELL1 versus BMP2 in osteogenesis and adipogenesis in vitro. Acta Biochim Biophys Sin (Shanghai). 44:856–865. 2012. View Article : Google Scholar