Zipper-interacting protein kinase interacts with human cell division cycle 14A phosphatase

Wu,Wei; Hu,Haiying; Ye,Zi; Leong,Mancheong; He,Min; Li,Qin; Hu,Renming; Zhang,Shuo

doi:10.3892/mmr.2014.3067

Zipper-interacting protein kinase interacts with human cell division cycle 14A phosphatase

Authors:
- Wei Wu
- Haiying Hu
- Zi Ye
- Mancheong Leong
- Min He
- Qin Li
- Renming Hu
- Shuo Zhang
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Affiliations: Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology at Fudan University, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Endocrinology and Metabolism, International Healthcare Center, Second Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Department of Endocrinology, City University of Macau, Macau 000853, P.R. China, Department of Endocrinology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200000, P.R. China
Published online on: December 9, 2014 https://doi.org/10.3892/mmr.2014.3067
Pages: 2775-2780

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Abstract

Zipper‑interacting protein kinase (ZIPK) is a novel serine/threonine protein kinase and a member of a large family of protein kinases, known as the death‑associated protein kinases. However, the function of ZIPK has yet to be fully elucidated, as few physiological substrates have currently been identified. In the present study, a yeast two‑hybrid screen was used and the human cell division cycle 14A (HsCdc14A) phosphatase was identified as a novel ZIPK binding protein. To the best of our knowledge, this is the first study to report the interaction between these proteins. The interaction between ZIPK and HsCdc14A was confirmed by in vitro experiments. In addition, ZIPK‑mediated phosphorylation was shown to activate the phosphatase activity of HsCdc14A. These findings indicated that ZIPK may also be involved in the regulation of the cell cycle in human cells, by interacting with HsCdc14A.

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