Effect of oxidized low-density lipoprotein on the expression of the prorenin receptor in human aortic smooth muscle cells

  • Authors:
    • Yunpeng Cheng
    • Xiaoyun Liu
    • Lijiao Zhang
    • Ying Zhang
    • Ying Liu
    • Yan Lu
    • Yinong Jiang
  • View Affiliations

  • Published online on: January 26, 2015     https://doi.org/10.3892/mmr.2015.3254
  • Pages: 4341-4344
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The activation of the (pro)renin receptor (PRR) may be potentially active in the development of atherosclerotic plaques independent of angiotensin II. Our previous studies demonstrated that high glucose was able to induce the activation of PRR. The present study was designed to determine the function of oxidized low‑density lipoprotein (ox‑LDL) on the expression of PRR in human aortic smooth muscle cells (HASMCs). Immunofluorescence revealed that PRR was expressed in HASMCs. HASMCs were cultured with 100 µg/ml ox‑LDL for 1, 2, 4, 6, 12 and 24 h, respectively. Subsequently, HASMCs were cultured with 25, 50, 100, 150, 200 and 300 µg/ml ox‑LDL for 6 h, respectively. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis revealed that the expression of PRR was markedly upregulated in a time‑ and concentration‑dependent manner, which peaked at 6 h and 50 µg/ml, then slowly decreased. Therefore, PRR may contribute to the atherogenesis effect induced by ox‑LDL.
View Figures
View References

Related Articles

Journal Cover

June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Cheng Y, Liu X, Zhang L, Zhang Y, Liu Y, Lu Y and Jiang Y: Effect of oxidized low-density lipoprotein on the expression of the prorenin receptor in human aortic smooth muscle cells. Mol Med Rep 11: 4341-4344, 2015
APA
Cheng, Y., Liu, X., Zhang, L., Zhang, Y., Liu, Y., Lu, Y., & Jiang, Y. (2015). Effect of oxidized low-density lipoprotein on the expression of the prorenin receptor in human aortic smooth muscle cells. Molecular Medicine Reports, 11, 4341-4344. https://doi.org/10.3892/mmr.2015.3254
MLA
Cheng, Y., Liu, X., Zhang, L., Zhang, Y., Liu, Y., Lu, Y., Jiang, Y."Effect of oxidized low-density lipoprotein on the expression of the prorenin receptor in human aortic smooth muscle cells". Molecular Medicine Reports 11.6 (2015): 4341-4344.
Chicago
Cheng, Y., Liu, X., Zhang, L., Zhang, Y., Liu, Y., Lu, Y., Jiang, Y."Effect of oxidized low-density lipoprotein on the expression of the prorenin receptor in human aortic smooth muscle cells". Molecular Medicine Reports 11, no. 6 (2015): 4341-4344. https://doi.org/10.3892/mmr.2015.3254