Elevated gene expression of S100A12 is correlated with the predominant clinical inflammatory factors in patients with bacterial pneumonia

Hou,Fei; Wang,Likui; Wang,Hong; Gu,Junchao; Li,Meiling; Zhang,Jingkai; Ling,Xiao; Gao,Xiaofang; Luo,Cheng

doi:10.3892/mmr.2015.3295

Elevated gene expression of S100A12 is correlated with the predominant clinical inflammatory factors in patients with bacterial pneumonia

Authors:
- Fei Hou
- Likui Wang
- Hong Wang
- Junchao Gu
- Meiling Li
- Jingkai Zhang
- Xiao Ling
- Xiaofang Gao
- Cheng Luo
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Affiliations: Department of Infection, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang, Beijing 100101, P.R. China, Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China, Key Laboratory of Food Nutrition and Safety, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Binhai, Tianjin 300457, P.R. China
Published online on: February 4, 2015 https://doi.org/10.3892/mmr.2015.3295
Pages: 4345-4352

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Abstract

Inflammation is the predominant characteristic of pneumonia. The present study aimed to to identify a faster and more reliable novel inflammatory marker for the diagnosis of pneumonia. The expression of the S100A12 gene was analyzed by reverse transcription quantitative polymerase chain reaction in samples obtained from 46 patients with bacterial pneumonia and other infections, compared with samples from 20 healthy individuals, using the 2‑ΔΔCt method. The expression levels of S100A12 were increased in 12 patients with bacterial pneumonia. Compared with clinical inflammatory data, a positive correlation was observed between the expression of the S100A12 gene and levels of white blood cells, C‑reactive protein (CRP), thrombocytocrit, neutrophils, erythrocyte sedimentation and soterocytes, and an inverse correlation was observed with the width of red blood cell volume distribution and platelet distribution, monocytes and hemoglobin, using Pearson's product‑moment correlation method. The P‑value of CRP and erythrocyte sedimentation were revealed to be statistically significant (P<0.05). A sporadic distribution of S100A12 was observed in a heatmap among the patients with different infections and bacterial pneumonia. Furthermore, the expression of S100A12 occurred in parallel to the number of clumps of inflamed tissue observed in chest computed tomography and X‑ray. The value of gene expression of S100A12 (>1.0) determined using the 2‑ΔΔCt method was associated with more severe respiratory diseases in the patients compromised by bacterial pneumonia, sepsis and pancreatitis. These findings suggested that S100A12 is an effective marker for inflammatory diseases.

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1	Maletzki C, Bodammer P, Breitruck A and Kerkhoff C: S100 proteins as diagnostic and prognostic markers in colorectal and hepatocellular carcinoma. Hepat Mon. 12:e72402012.PubMed/NCBI
2	Schafer BW, Wicki R, Engelkamp D, Mattei MG and Heizmann CW: Isolation of a YAC clone covering a cluster of nine S100 genes on human chromosome 1q21: rationale for a new nomenclature of the S100 calcium-binding protein family. Genomics. 25:638–643. 1995. View Article : Google Scholar : PubMed/NCBI
3	Yamamura T, Hitomi J, Nagasaki K, et al: Human CAAF1 gene - molecular cloning, gene structure, and chromosome mapping. Biochem Biophys Res Commun. 221:356–360. 1996. View Article : Google Scholar : PubMed/NCBI
4	Roth J, Vogl T, Sorg C and Sunderkotter C: Phagocyte-specific S100 proteins: a novel group of proinflammatory molecules. Trends Immunol. 24:155–158. 2003. View Article : Google Scholar : PubMed/NCBI
5	Guignard F, Mauel J and Markert M: Identification and characterization of a novel human neutrophil protein related to the S100 family. Biochem J. 309:395–401. 1995.PubMed/NCBI
6	Marti T, Erttmann KD and Gallin MY: Host-parasite interaction in human onchocerciasis: identification and sequence analysis of a novel human calgranulin. Biochem Biophys Res Commun. 221:454–458. 1996. View Article : Google Scholar : PubMed/NCBI
7	Ryckman C, Robichaud GA, Roy J, Cantin R, Tremblay MJ and Tessier PA: HIV-1 transcription and virus production are both accentuated by the proinflammatory myeloid-related proteins in human CD4+ T lymphocytes. J Immunol. 169:3307–3313. 2002. View Article : Google Scholar : PubMed/NCBI
8	Moroz OV, Antson AA, Dodson GG, et al: Crystallization and preliminary X-ray diffraction analysis of human calcium-binding protein S100A12. Acta Crystallogr D Biol Crystallogr. 56:189–191. 2000. View Article : Google Scholar : PubMed/NCBI
9	Ilg EC, Troxler H, Burgisser DM, et al: Amino acid sequence determination of human S100A12 (P6, calgranulin C, CGRP, CAAF1) by tandem mass spectrometry. Biochem Biophys Res Commun. 225:146–150. 1996. View Article : Google Scholar : PubMed/NCBI
10	Cole AM, Kim YH, Tahk S, et al: Calcitermin, a novel antimicrobial peptide isolated from human airway secretions. FEBS Lett. 504:5–10. 2001. View Article : Google Scholar : PubMed/NCBI
11	Filipek A, Jastrzebska B, Nowotny M and Kuznicki J: CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family. J Biol Chem. 277:28848–28852. 2002. View Article : Google Scholar : PubMed/NCBI
12	Morbini P, Villa C, Campo I, Zorzetto M, Inghilleri S and Luisetti M: The receptor for advanced glycation end products and its ligands: a new inflammatory pathway in lung disease. Mod Pathol. 19:1437–1445. 2006.PubMed/NCBI
13	Kikkawa T, Sato N, Kojika M, et al: Significance of measuring S100A12 and sRAGE in the serum of sepsis patients with postoperative acute lung injury. Dig Surg. 27:307–312. 2010. View Article : Google Scholar : PubMed/NCBI
14	Hofmann MA, Drury S, Fu C, et al: RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides. Cell. 97:889–901. 1999. View Article : Google Scholar : PubMed/NCBI
15	Moroz OV, Antson AA, Murshudov GN, et al: The three-dimensional structure of human S100A12. Acta Crystallogr D Biol Crystallogr. 57:20–29. 2001. View Article : Google Scholar : PubMed/NCBI
16	Moroz OV, Antson AA, Dodson EJ, et al: The structure of S100A12 in a hexameric form and its proposed role in receptor signalling. Acta Crystallogr D Biol Crystallogr. 58:407–413. 2002. View Article : Google Scholar : PubMed/NCBI
17	Machelska H, Schopohl JK, Mousa SA, Labuz D, Schafer M and Stein C: Different mechanisms of intrinsic pain inhibition in early and late inflammation. J Neuroimmunol. 141:30–39. 2003. View Article : Google Scholar : PubMed/NCBI
18	Stein C, Schafer M and Machelska H: Attacking pain at its source: new perspectives on opioids. Nat Med. 9:1003–1008. 2003. View Article : Google Scholar : PubMed/NCBI
19	Medzhitov R: Origin and physiological roles of inflammation. Nature. 454:428–435. 2008. View Article : Google Scholar : PubMed/NCBI
20	Spiekerkoetter E, Guignabert C, de Jesus Perez V, et al: S100A4 and bone morphogenetic protein-2 codependently induce vascular smooth muscle cell migration via phospho-extracellular signal-regulated kinase and chloride intracellular channel 4. Circ Res. 105:639–647. 2009. View Article : Google Scholar : PubMed/NCBI
21	Dabritz J, Langhorst J, Lugering A, et al: Improving relapse prediction in inflammatory bowel disease by neutrophil-derived S100A12. Inflamm Bowel Dis. 19:1130–1138. 2013. View Article : Google Scholar : PubMed/NCBI
22	Hofmann BMA, Heydemann A, Gawdzik J, Shilling RA and Camoretti-Mercado B: Transgenic expression of human S100A12 induces structural airway abnormalities and limited lung inflammation in a mouse model of allergic inflammation. Clin Exp Allergy. 41:878–889. 2011. View Article : Google Scholar
23	van Zoelen MA, Achouiti A and van der Poll T: RAGE during infectious diseases. Front Biosci (Schol Ed). 3:1119–1132. 2011. View Article : Google Scholar
24	Batliwalla FM, Baechler EC, Xiao X, et al: Peripheral blood gene expression profiling in rheumatoid arthritis. Genes Immun. 6:388–397. 2005. View Article : Google Scholar : PubMed/NCBI
25	Das D, Gawdzik J, Dellefave-Castillo L, McNally EM, Husain A, et al: S100A12 expression in thoracic aortic aneurysm is associated with increased risk of dissection and perioperative complications. J Am Coll Cardiol. 60:775–785. 2012. View Article : Google Scholar : PubMed/NCBI
26	Brinar M, Cleynen I, Coopmans T, et al: Serum S100A12 as a new marker for inflammatory bowel disease and its relationship with disease activity. Gut. 59:1728–1729. 2010. View Article : Google Scholar : PubMed/NCBI
27	Dabritz J, Jenke A, Wirth S and Foell D: Fecal phagocyte-specific S100A12 for diagnosing necrotizing enterocolitis. J Pediatr. 161:1059–1064. 2012. View Article : Google Scholar : PubMed/NCBI
28	Nakashima M, Sakai T, Hiraiwa H, et al: Role of S100A12 in the pathogenesis of osteoarthritis. Biochem Biophys Res Commun. 422:508–514. 2012. View Article : Google Scholar : PubMed/NCBI
29	Foell D, Ichida F, Vogl T, et al: S100A12 (EN-RAGE) in monitoring Kawasaki disease. Lancet. 361:1270–1272. 2003. View Article : Google Scholar : PubMed/NCBI
30	Foell D, Kucharzik T, Kraft M, et al: Neutrophil derived human S100A12 (EN-RAGE) is strongly expressed during chronic active inflammatory bowel disease. Gut. 52:847–853. 2003. View Article : Google Scholar : PubMed/NCBI
31	Ginocchio CC, Lotlikar M, Falk L, et al: Clinical performance of the 3M Rapid Detection Flu A+B Test compared to R-Mix culture, DFA and BinaxNOW Influenza A&B Test. J Clin Virol. 45:146–149. 2009. View Article : Google Scholar : PubMed/NCBI
32	Rongeat N, Ledroit S, Chauvet L, et al: Automatic cytometric device using multiple wavelength excitations. J Biomed Opt. 16:0570062011. View Article : Google Scholar : PubMed/NCBI
33	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar
34	Braun RP and Lee JS: Immunogenic duplex nucleic acids are nuclease resistant. J Immunol. 141:2084–2089. 1988.PubMed/NCBI
35	Smyth GK: Limma: linear models for microarray data. Bioinformatics and Computational Biology Solutions using R and Bioconductor. Gentleman R, Carey V, Dut SR, Irizarry WH and Huber W: Springer; New York: pp. 397–420. 2005, View Article : Google Scholar
36	Pradeep AR, Martande SS, Singh SP, et al: Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes. Inflamm Res. 63:317–323. 2014. View Article : Google Scholar : PubMed/NCBI
37	Dellinger RP, Levy MM, Rhodes A, et al: Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock, 2012. Intensive Care Med. 39:165–228. 2013. View Article : Google Scholar : PubMed/NCBI
38	Gaïni S, Koldkjaer OG, Pedersen C and Pedersen SS: Procalcitonin, lipopolysaccharide-binding protein, interleukin-6 and C-reactive protein in community-acquired infections and sepsis: a prospective study. Critical care. 10:R532006. View Article : Google Scholar
39	Ballou SP and Kushner I: C-reactive protein and the acute phase response. Adv Intern Med. 37:313–336. 1992.PubMed/NCBI
40	Pietzsch J and Hoppmann S: Human S100A12: a novel key player in inflammation. Amino Acids. 36:381–389. 2009. View Article : Google Scholar