Association of hsa-miR‑145 overexpression in human testicular cells with male infertility

  • Authors:
    • Liyuan Zhang
    • Xianping Ding
    • Shuangshuang Nie
    • Jesse Li‑Ling
    • Yuping Zhang
    • Hui Zhang
    • Lin Chen
    • Lingxiao Li
    • Min Ding
  • View Affiliations

  • Published online on: January 29, 2015     https://doi.org/10.3892/mmr.2015.3273
  • Pages: 4365-4372
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Abstract

MicroRNAs (miRs) have crucial functions in spermatogenesis and implications for male infertility. In the present study, Homo sapiens (hsa)‑miR‑145 was designed and cloned into the eukaryotic expression plasmid pGenesil‑1. The recombinant plasmids were transfected into Hs 1.tes normal testicular cells and NTERA‑2 testicular cancer cells. Quantitative polymerase chain reaction of hsa‑miR‑145 indicated that pGenesil‑1‑miR‑145 effectively upregulated the expression of hsa‑miR‑145 in vitro. hsa‑miR‑145 overexpression inhibited the mRNA and protein expression of sex-determining region Y Box 9 in Hs 1.tes cells. The proliferation rates of NTERA‑2 cells transfected with pGenesil‑1‑miR‑145 were significantly decreased. High expression levels of miR‑145 promoted cell apoptosis in NTERA‑2 cells. The results revealed that altered hsa‑miR‑145 expression in testicular cells affects the regulation of target genes associated with male infertility.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang L, Ding X, Nie S, Li‑Ling J, Zhang Y, Zhang H, Chen L, Li L and Ding M: Association of hsa-miR‑145 overexpression in human testicular cells with male infertility. Mol Med Rep 11: 4365-4372, 2015.
APA
Zhang, L., Ding, X., Nie, S., Li‑Ling, J., Zhang, Y., Zhang, H. ... Ding, M. (2015). Association of hsa-miR‑145 overexpression in human testicular cells with male infertility. Molecular Medicine Reports, 11, 4365-4372. https://doi.org/10.3892/mmr.2015.3273
MLA
Zhang, L., Ding, X., Nie, S., Li‑Ling, J., Zhang, Y., Zhang, H., Chen, L., Li, L., Ding, M."Association of hsa-miR‑145 overexpression in human testicular cells with male infertility". Molecular Medicine Reports 11.6 (2015): 4365-4372.
Chicago
Zhang, L., Ding, X., Nie, S., Li‑Ling, J., Zhang, Y., Zhang, H., Chen, L., Li, L., Ding, M."Association of hsa-miR‑145 overexpression in human testicular cells with male infertility". Molecular Medicine Reports 11, no. 6 (2015): 4365-4372. https://doi.org/10.3892/mmr.2015.3273