Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis

  • Authors:
    • Rong‑Ping Zhou
    • Ming‑Tao Deng
    • Lan‑Ying Chen
    • Ning Fang
    • Chuan Du
    • Lin‑Pan Chen
    • Ye‑Qing Zou
    • Jiang‑Hua Dai
    • Mei‑Lan Zhu
    • Wei Wang
    • Si‑Jian Lin
    • Rong‑Hua Liu
    • Jun Luo
  • View Affiliations

  • Published online on: January 30, 2015     https://doi.org/10.3892/mmr.2015.3285
  • Pages: 4489-4495
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Abstract

Chlorogenic acid (CGA) exhibits various biological properties, including the inhibition of oxidation, obesity, apoptosis and tumorigenesis. CGA is also able to promote cell survival and proliferation. The aim of the present study was to determine the effects and underlying molecular mechanisms of CGA on the adipogenesis of bone marrow‑derived mesenchymal stem cells (BMSCs). Treatment with CGA had a marginal effect on cell proliferation, by promoting the expression levels of phosphorylated Akt and cyclin D1. Furthermore, treatment with CGA also upregulated the phosphorylation of extracellular signal‑regulated kinase (Erk) and inhibited the adipocyte differentiation of BMSCs by inhibiting the expression of peroxisome proliferator‑activated receptor (PPAR)γ and CCAAT/enhancer binding protein (C/EBP)α. However, knockdown of the expression of Shp2 attenuated CGA‑induced proliferation and inhibited the phosphorylation of Akt and expression of cyclin D1. Furthermore, CGA treatment upregulated Erk phosphorylation and decreased the expression levels of PPARγ and CEBPα, which was inhibited by treatment with the Shp2 PTPase activity inhibitor, NSC‑87877. The results of the present study suggested that CGA‑induced Akt and Erk pathways regulate proliferation and differentiation and that Shp2 is important in the proliferation and differentiation of BMSCs.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou RP, Deng MT, Chen LY, Fang N, Du C, Chen LP, Zou YQ, Dai JH, Zhu ML, Wang W, Wang W, et al: Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis. Mol Med Rep 11: 4489-4495, 2015.
APA
Zhou, R., Deng, M., Chen, L., Fang, N., Du, C., Chen, L. ... Luo, J. (2015). Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis. Molecular Medicine Reports, 11, 4489-4495. https://doi.org/10.3892/mmr.2015.3285
MLA
Zhou, R., Deng, M., Chen, L., Fang, N., Du, C., Chen, L., Zou, Y., Dai, J., Zhu, M., Wang, W., Lin, S., Liu, R., Luo, J."Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis". Molecular Medicine Reports 11.6 (2015): 4489-4495.
Chicago
Zhou, R., Deng, M., Chen, L., Fang, N., Du, C., Chen, L., Zou, Y., Dai, J., Zhu, M., Wang, W., Lin, S., Liu, R., Luo, J."Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis". Molecular Medicine Reports 11, no. 6 (2015): 4489-4495. https://doi.org/10.3892/mmr.2015.3285