Activin A regulates proliferation, invasion and migration in osteosarcoma cells

  • Authors:
    • Jianwei Zhu
    • Fan Liu
    • Quanming Wu
    • Xiancheng Liu
  • View Affiliations

  • Published online on: January 30, 2015     https://doi.org/10.3892/mmr.2015.3284
  • Pages: 4501-4507
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Abstract

Activin A is a member of the TGF‑β superfamily. Previous studies have demonstrated that activin A exhibited pluripotent effects in several tumours. However, the roles of activin A signaling in osteosarcoma pathogenesis have not been previously investigated. Therefore, the present study aimed to investigate the effects of activin A on osteosarcoma cell proliferation, invasion and migration. Firstly, the expression of activin A in osteosarcoma cell lines (MG63, SaOS‑2 and U2OS) and a human osteoblastic cell line (hFOB1.19) was detected using reverse transcription quantitative polymerase chain reaction and western blotting. Activin A was upregulated in osteosarcoma cell lines compared with hFOB1.19 cells. To investigate the effects of activin A on osteosarcoma cell proliferation, invasion and migration, MG63 cells were generated in which activin A was either overexpressed or depleted. MTT staining, propidium iodide staining and a Transwell assay were used to analyze the cell cycle, proliferation, invasion and migration of MG63 cells, respectively. The results of the present study revealed that the abilities of proliferation, invasion and migration were suppressed in MG63 cells in which activin A was depleted, while they were enhanced in activin A-overexpressing cells. In conclusion, the results of the present study suggested that activin A may facilitate proliferation, invasion and migration of osteosarcoma cells, and it may therefore be a potential target for the treatment of osteosarcoma.
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June-2015
Volume 11 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Zhu J, Liu F, Wu Q and Liu X: Activin A regulates proliferation, invasion and migration in osteosarcoma cells. Mol Med Rep 11: 4501-4507, 2015.
APA
Zhu, J., Liu, F., Wu, Q., & Liu, X. (2015). Activin A regulates proliferation, invasion and migration in osteosarcoma cells. Molecular Medicine Reports, 11, 4501-4507. https://doi.org/10.3892/mmr.2015.3284
MLA
Zhu, J., Liu, F., Wu, Q., Liu, X."Activin A regulates proliferation, invasion and migration in osteosarcoma cells". Molecular Medicine Reports 11.6 (2015): 4501-4507.
Chicago
Zhu, J., Liu, F., Wu, Q., Liu, X."Activin A regulates proliferation, invasion and migration in osteosarcoma cells". Molecular Medicine Reports 11, no. 6 (2015): 4501-4507. https://doi.org/10.3892/mmr.2015.3284