α-tomatine inhibits growth and induces apoptosis in HL-60 human myeloid leukemia cells

Huang,Huarong; Chen,Shaohua; Van Doren,Jeremiah; Li,Dongli; Farichon,Chelsea; He,Yan; Zhang,Qiuyan; Zhang,Kun; Conney,Allan  H.; Goodin,Susan; Du,Zhiyun; Zheng,Xi

doi:10.3892/mmr.2015.3238

α-tomatine inhibits growth and induces apoptosis in HL-60 human myeloid leukemia cells

Authors:
- Huarong Huang
- Shaohua Chen
- Jeremiah Van Doren
- Dongli Li
- Chelsea Farichon
- Yan He
- Qiuyan Zhang
- Kun Zhang
- Allan H. Conney
- Susan Goodin
- Zhiyun Du
- Xi Zheng
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Affiliations: Allan H. Conney Laboratory for Anticancer Research, Guangdong University of Technology, Guangzhou, Guangdong 510006, P.R. China, Department of Otolaryngology, Guangdong Provincial People's Hospital, Guangzhou, Guangdong 510006, P.R. China, Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA, Division of Medical Oncology, Rutgers Cancer Institute of New Jersey, New Brunswick, NJ 08903, USA
Published online on: January 22, 2015 https://doi.org/10.3892/mmr.2015.3238
Pages: 4573-4578

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Abstract

α‑tomatine is a glycoalkaloid that occurs naturally in tomatoes (Lycopersicon esculentum). In the present study, the effects of α‑tomatine on human myeloid leukemia HL‑60 cells were investigated. Treatment of HL‑60 cells with α‑tomatine resulted in growth inhibition and apoptosis in a concentration‑dependent manner. Tomatidine, the aglycone of tomatine had little effect on the growth and apoptosis of HL‑60 cells. Growth inhibition and apoptosis induced by α‑tomatine in HL‑60 cells was partially abrogated by addition of cholesterol indicating that interactions between α‑tomatine and cell membrane‑associated cholesterol may be important in mediating the effect of α‑tomatine. Activation of nuclear factor‑κB by the phorbol ester, 12‑O‑tetradecanoylphorbol‑13‑acetate failed to prevent apoptosis in HL‑60 cells treated with α‑tomatine. In animal experiments, it was found that treatment of mice with α‑tomatine inhibited the growth of HL‑60 xenografts in vivo. Results from the present study indicated that α‑tomatine may have useful anti‑leukemia activities.

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