Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers

  • Authors:
    • Zi-Qiang Zhu
    • Jin-Shan Tang
    • Duan Gang
    • Ming-Xing Wang
    • Jian-Qiang Wang
    • Zhou Lei
    • Zhou Feng
    • Ming-Liang Fang
    • Lin Yan
  • View Affiliations

  • Published online on: March 24, 2015     https://doi.org/10.3892/mmr.2015.3535
  • Pages: 1157-1162
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Abstract

The aim of the present study was to identify biomarkers in osteosarcoma (OS) cell serum by antibody microarray profiling, which may be used for OS diagnosis and therapy. An antibody microarray was used to detect the expression levels of cytokines in serum samples from 20 patients with OS and 20 healthy individuals. Significantly expressed cytokines in OS serum were selected when P<0.05 and fold change >2. An enzyme-linked immunosorbent assay (ELISA) was used to validate the antibody microarray results. Finally, classification accuracy was calculated by cluster analysis. Twenty one cytokines were significantly upregulated in OS cell serum samples compared with control samples. Expression of inteleukin‑6, monocyte chemoattractant protein‑1, tumor growth factor‑β, growth-related oncogene, hepatocyte grwoth factor, chemokine ligand 16, Endoglin, matrix metalloproteinase‑9 and platelet-derived growth factor-AA was validated by ELISAs. OS serum samples and control samples were distinguished by significantly expressed cytokines with an accuracy of 95%. The results demonstrated that expressed cytokines identified by antibody microarray may be used as biomarkers for OS diagnosis and therapy.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu Z, Tang J, Gang D, Wang M, Wang J, Lei Z, Feng Z, Fang M and Yan L: Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers. Mol Med Rep 12: 1157-1162, 2015
APA
Zhu, Z., Tang, J., Gang, D., Wang, M., Wang, J., Lei, Z. ... Yan, L. (2015). Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers. Molecular Medicine Reports, 12, 1157-1162. https://doi.org/10.3892/mmr.2015.3535
MLA
Zhu, Z., Tang, J., Gang, D., Wang, M., Wang, J., Lei, Z., Feng, Z., Fang, M., Yan, L."Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers". Molecular Medicine Reports 12.1 (2015): 1157-1162.
Chicago
Zhu, Z., Tang, J., Gang, D., Wang, M., Wang, J., Lei, Z., Feng, Z., Fang, M., Yan, L."Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers". Molecular Medicine Reports 12, no. 1 (2015): 1157-1162. https://doi.org/10.3892/mmr.2015.3535