Comparative analysis of the influence of Fructus Ligustri Lucidi on a rat lumbar disc herniation model

Han,Ya‑Xin; Liang,Dong; Han,Xiao‑Rui; Liang,De‑Yong

doi:10.3892/mmr.2015.3547

Comparative analysis of the influence of Fructus Ligustri Lucidi on a rat lumbar disc herniation model

Authors:
- Ya‑Xin Han
- Dong Liang
- Xiao‑Rui Han
- De‑Yong Liang
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Affiliations: Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: March 24, 2015 https://doi.org/10.3892/mmr.2015.3547
Pages: 1225-1232

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Abstract

Lumbar disc herniation (LDH) is a term used for a group of conditions, including back pain, femoral nerve pain and sciatica. Currently available treatments and surgical options are insufficient for patients with LDH. Fructus Ligustri Lucidi (FLL) is a herb that is used for treating age‑associated diseases. The results of the present study suggested that FLL may be used for treatment of patients with LDH. In the present study, matrix metalloproteinase‑1, ‑3, ‑8 and ‑9 (MMP‑1, ‑3, ‑8 and ‑9) protein and mRNA expression downregulation was observed in patients with LDH according to western blotting and reverse transcription‑quantitative polymerase chain reaction. By contrast, upregulation of interleukin‑2 (IL‑2), IL‑6, IL‑8 and tumor necrosis factor‑α (TNF‑α) expression was observed in patients with LDH, according to an enzyme‑linked immunosorbent assay. Mechanical allodynia was observed in rats with LDH not treated with FLL; however, not in FLL‑treated rats. IL‑2, IL‑6, IL‑8 and TNF‑α expression levels in the serum from untreated rats were significantly higher than that of the FLL‑treated rat models. Protein expression levels of MMPs in FLL‑treated rats were lower than those in untreated rats. However, the mechanisms underlying the association between FLL and protein expression levels require further investigation.

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