High-mobility group nucleosome-binding domain 2 protein inhibits the invasion of Klebsiella pneumoniae into mouse lungs in vivo

Zheng,Shuang; Ren,Laibin; Li,Heng; Shen,Xiaofei; Yang,Xiaolong; Li,Na; Wang,Xinyuan; Guo,Xiaojuan; Wang,Xiaoying; Huang,Ning

doi:10.3892/mmr.2015.3464

High-mobility group nucleosome-binding domain 2 protein inhibits the invasion of Klebsiella pneumoniae into mouse lungs in vivo

Authors:
- Shuang Zheng
- Laibin Ren
- Heng Li
- Xiaofei Shen
- Xiaolong Yang
- Na Li
- Xinyuan Wang
- Xiaojuan Guo
- Xiaoying Wang
- Ning Huang
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Affiliations: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: March 10, 2015 https://doi.org/10.3892/mmr.2015.3464
Pages: 1279-1285

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Abstract

Since bacterial invasion into host cells is a critical step in the infection process and the predominance of multiple‑antibiotic‑resistant Klebsiella (K.) pneumoniae strains, using molecular agents to interfere with K. pneumoniae invasion is an attractive approach for the prevention of infection and suppress the immune inflammatory response. In previous studies by our group, high‑mobility group nucleosome‑binding domain 2 (HMGN2) protein was shown to exhibit anti‑bacterial activity in vitro. The objective of the present study was to investigate the effects of HMGN2 protein on the invasion of K. pneumoniae 03183 in vivo. The results showed that pre‑treatment with 128 µg/ml HMGN2 significantly reduced K. pneumoniae 03183 invasion into mouse lungs and increased the mRNA expression of CXCL1 and LCN2 within 2 h. Immunohistochemical staining showed that F‑actin expression was significantly decreased, and fluorescence microscopy and western blot analysis further demonstrated that HMGN2 significantly blocked K. pneumoniae 03183‑induced actin polymerization. These changes implied that HMGN2 may provide protection against K. pneumoniae 03183 infection in vivo.

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