Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains Corrigendum in /mmr/12/3/3998

Hu,Hai  Xia; Lin,Ru  Hui; Zhu,Xiao  Qin; Li,Zuan  Fang; Chen,Li  Dian

doi:10.3892/mmr.2015.3511

Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains

Corrigendum in: /mmr/12/3/3998

Authors:
- Hai Xia Hu
- Ru Hui Lin
- Xiao Qin Zhu
- Zuan Fang Li
- Li Dian Chen
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Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China, College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China
Published online on: March 19, 2015 https://doi.org/10.3892/mmr.2015.3511
Pages: 1321-1327

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Abstract

The aim of the present study was to explore the neuroprotective effects of Gualou Guizhi decoction (GLGZD) in a rat model of middle cerebral artery occlusion (MCAO). Sprague‑Dawley rats were divided into three groups: Sham (no MCAO), MCAO (MCAO with no GLGZD treatment) and GLGZD (MCAO with GLGZD treatment). Rats in the MCAO and GLGZD groups were subjected to permanent occlusion of the left middle cerebral artery. Neurological function and infarct volume were measured. Microglial activation and inflammatory cell accumulation were measured using immunohistochemistry. mRNA and protein expression of inflammatory mediators were examined using reverse transcription-quantitative polymerase chain reaction and an enzyme‑linked immunosorbent assay. The expression of proteins associated with the nuclear factor κ‑B (NF‑κB) inflammation signaling pathway was analyzed using western blotting. The results of the present study suggested that infarct size was significantly reduced and neurological behavior function was improved in rats with MCAO treated with GLGZD compared with rats in the MCAO group. Amoeboid microglial expansion and inflammatory cell migration were observed in the infarcted areas of rats in the GLGZD group and were not identified in those of the MCAO group. Target mRNA and protein levels, and inflammatory cell infiltration were significantly reduced in the GLGZD group compared with the MCAO model group. Notably, GLGZD treatment induced neuroprotective effects, reducing inflammation and inhibiting NF‑κB signaling compared with the MCAO group. Therefore, GLGZD may exhibit anti‑inﬂammatory effects against ischemia‑reperfusion brain injury and may be a therapeutic target for ischemic stroke.

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1	Lloyd-Jones D, Adams R, Carnethon M, De Simone G, Ferguson TB, Flegal K, Ford E, Furie K, Go A, Greenlund K, et al: American Heart Association Statistics Committee and Stroke Statistics Subcommittee: Heart disease and stroke statistics - 2009 update: A report from the American heart association statistics committee and stroke statistics subcommittee. Circulation. 119:e21–e181. 2009. View Article : Google Scholar
2	Durai Pandian J, Padma V, Vijaya P, Sylaja PN and Murthy JM: Stroke and thrombolysis in developing countries. Int J Stroke. 2:17–26. 2007. View Article : Google Scholar
3	Sims NR and Muyderman H: Mitochondria, oxidative metabolism and cell death in stroke. Biochim Biophys Acta. 1802:80–91. 2010. View Article : Google Scholar
4	Hishida R, Kamatani D, Kitaura H, Kudoh M and Shibuki K: Functional local connections with differential activity-dependence and critical periods surrounding the primary auditory cortex in rat cerebral slices. Neuroimage. 34:679–693. 2007. View Article : Google Scholar
5	Ginsberg LD: Impact of drug tolerability on the selection of antidepressant treatment in patients with major depressive disorder. CNS Spectr. 14(Suppl 12): 8–14. 2009.
6	Tuma RF and Steffens S: Targeting the endocannabinod system to limit myocardial and cerebral ischemic and reperfusion injury. Curr Pharm Biotechnol. 13:46–58. 2012. View Article : Google Scholar
7	Kim HJ and Chuang DM: HDAC inhibitors mitigate ischemia-induced oligodendrocyte damage: Potential roles of oligodendrogenesis, VEGF, and anti-inflammation. Am J Transl Res. 6:206–223. 2014.PubMed/NCBI
8	Xie L, Sun F, Wang J, Mao X, Xie L, Yang SH, Su DM, Simpkins JW, Greenberg DA and Jin K: mTOR signaling inhibition modulates macrophage/microglia-mediated neuro-inflammation and secondary injury via regulatory T cells after focal ischemia. J Immunol. 192:6009–6019. 2014. View Article : Google Scholar : PubMed/NCBI
9	Sheridan GK and Murphy KJ: Neuron-glia crosstalk in health and disease: Fractalkine and CX3CR1 take centre stage. Open Biol. 3:1301812013. View Article : Google Scholar : PubMed/NCBI
10	Vartanian KB, Stevens SL, Marsh BJ, Williams-Karnesky R, Lessov NS and Stenzel-Poore MP: LPS preconditioning redirects TLR signaling following stroke: TRIF-IRF3 plays a seminal role in mediating tolerance to ischemic injury. J Neuroinflammation. 8:1402011. View Article : Google Scholar : PubMed/NCBI
11	Sladojevic N, Stamatovic SM, Keep RF, Grailer JJ, Sarma JV, Ward PA and Andjelkovic AV: Inhibition of junctional adhesion molecule-A/LFA interaction attenuates leukocyte trafficking and inflammation in brain ischemia/reperfusion injury. Neurobiol Dis. 67:57–70. 2014. View Article : Google Scholar : PubMed/NCBI
12	Chen S, Yin ZJ, Jiang C, Ma ZQ, Fu Q, Qu R and Ma SP: Asiaticoside attenuates memory impairment induced by transient cerebral ischemia-reperfusion in mice through anti-inflammatory mechanism. Pharmacol Biochem Behav. 122:7–15. 2014. View Article : Google Scholar : PubMed/NCBI
13	Liu Y, Lian Z, Zhu H, Wang Y, Yu S, Chen T, Qu J, Li J, Ma S and Chen X: A systematic, integrated study on the neuroprotective effects of hydroxysafflor yellow A revealed by (1)H NMR-based metabonomics and the NF-κB pathway. Evid Based Complement Alternat Med. 2013:1473622013. View Article : Google Scholar
14	Zhang L and Ai H: Effects of Gua Lou Gui Zhi decoction on c-fos and c-jun on epileptic rats. Sichuan. J Tradit Chin Med. 23:21–22. 2005.In Chinese.
15	Yang C, Chen L and Tao J: New usage of a classical formula-Gua Lou Gui Zhi decoction. Liaoning J Tradit Chin Med. 39:1599–1600. 2012.In Chinese.
16	Huang J, Tao J, Xue X, Yang S, Han P, Lin Z, Xu W, Lin J, Peng J and Chen L: Gua Lou Gui Zhi decoction exerts neuroprotective effects on post-stroke spasticity via the modulation of glutamate levels and AMPA receptor expression. Int J Mol Med. 31:841–848. 2013.PubMed/NCBI
17	Hu H, Li Z, Zhu X, Lin R, Lin J, Peng J, Tao J and Chen L: Gua Lou Gui Zhi decoction suppresses LPS-induced activation of the TLR4/NF-κB pathway in BV-2 murine microglial cells. Int J Mol Med. 31:1327–1332. 2013.PubMed/NCBI
18	Hu H, Li Z, Zhu X, Lin R, Peng J, Tao J and Chen L: GuaLou GuiZhi decoction inhibits LPS-induced microglial cell motility through the MAPK signaling pathway. Int J Mol Med. 32:1281–1286. 2013.PubMed/NCBI
19	Xue X, You Y, Tao J, Ye X, Huang J, Yang S, Lin Z, Hong Z, Peng J and Chen L: Electro-acupuncture at points of Zusanli and Quchi exerts anti-apoptotic effect through the modulation of PI3K/Akt signaling pathway. Neurosci Lett. 558:14–19. 2014. View Article : Google Scholar
20	Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI
21	Jiang B, Wang WZ, Chen H, Hong Z, Yang QD, Wu SP, Du XL and Bao QJ: Incidence and trends of stroke and its subtypes in China: Results from three large cities. Stroke. 37:63–68. 2006. View Article : Google Scholar
22	Guo JM, Liu AJ and Su DF: Genetics of stroke. Acta Pharmacol Sin. 31:1055–1064. 2010. View Article : Google Scholar : PubMed/NCBI
23	Zhang L, Dong LY, Li YJ, Hong Z and Wei WS: The microRNA miR-181c controls microglia-mediated neuronal apoptosis by suppressing tumor necrosis factor. J Neuroinflammation. 9:2112012. View Article : Google Scholar : PubMed/NCBI
24	Lu X, Ma L, Ruan L, Kong Y, Mou H, Zhang Z, Wang Z, Wang JM and Le Y: Resveratrol differentially modulates inflammatory responses of microglia and astrocytes. J Neuroinflammation. 7:462010. View Article : Google Scholar : PubMed/NCBI
25	Kim JB, Yu YM, Kim SW and Lee JK: Anti-inflammatory mechanism is involved in ethyl pyruvate-mediated efficacious neuroprotection in the postischemic brain. Brain Res. 1060:188–192. 2005. View Article : Google Scholar : PubMed/NCBI
26	Perkins ND: Integrating cell-signalling pathways with NF-kappaB and IKK function. Nat Rev Mol Cell Biol. 8:49–62. 2007. View Article : Google Scholar
27	Guo RB, Wang GF, Zhao AP, Gu J, Sun XL and Hu G: Paeoniflorin protects against ischemia-induced brain damages in rats via inhibiting MAPKs/NF-κB-mediated inflammatory responses. PLoS One. 7:e497012012. View Article : Google Scholar
28	Dejda A, Seaborn T, Bourgault S, Touzani O, Fournier A, Vaudry H and Vaudry D: PACAP and a novel stable analog protect rat brain from ischemia: Insight into the mechanisms of action. Peptides. 32:1207–1216. 2011. View Article : Google Scholar : PubMed/NCBI
29	Gibson CL: Cerebral ischemic stroke: Is gender important? J Cereb Blood Flow Metab. 33:1355–1361. 2013. View Article : Google Scholar : PubMed/NCBI