Ophiopogonin‑D suppresses MDA‑MB‑435 cell adhesion and invasion by inhibiting matrix metalloproteinase‑9

Zhang,Yuanyuan; Han,Yuwei; Zhai,Kefeng; Sun,Minhui; Liu,Jihua; Yu,Boyang; Kou,Junping

doi:10.3892/mmr.2015.3541

Ophiopogonin‑D suppresses MDA‑MB‑435 cell adhesion and invasion by inhibiting matrix metalloproteinase‑9

Authors:
- Yuanyuan Zhang
- Yuwei Han
- Kefeng Zhai
- Minhui Sun
- Jihua Liu
- Boyang Yu
- Junping Kou
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Affiliations: Department of Complex Prescription of Traditional Chinese Medicine, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 211198, P.R. China
Published online on: March 24, 2015 https://doi.org/10.3892/mmr.2015.3541
Pages: 1493-1498

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Abstract

Ophiopogonin‑D is one of steroidal saponins isolated from the root of the Chinese medicinal plant Ophiopogon japonicas. It has been claimed to possess anti‑inflammatory and anti‑oxidant properties. The present study was the first to examine the anti‑tumor metastasis properties of ophiopogonin‑D. An MTT assay showed that ophiopogonin‑D inhibited the proliferation of MDA‑MB‑435 melanoma cells, and decreased invasion was demonstrated using a Transwell invasion assay. Furthermore, adhesion of MDA‑MB‑435 cells to human umbilical vascular endothelial cells and to fibronectin was inhibited by ophiopogonin‑D. Gelatin zymography and western blot analysis showed that ophiopogonin‑D inhibited the expression and secretion of matrix metalloproteinase‑9 (MMP‑9), but not that of MMP‑2. Inhibition of phosphorylation of p38 by ophiopogonin‑D indicated its inhibition of the mitogen‑activated protein kinase pathway. Overall, the results suggested that ophiopogonin‑D may be considered as a candidate drug for treating or preventing tumor metastasis.

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