Lentivirus-mediated knockdown of rhomboid domain containing 1 inhibits colorectal cancer cell growth

Han,Junyi; Bai,Junchao; Yang,Yao; Yin,Hua; Gao,Wei; Lu,Aiguo; Liu,Fei; Ge,Haiyan; Liu,Zhongmin; Wang,Jinyi; Zhong,Lan

doi:10.3892/mmr.2015.3365

Lentivirus-mediated knockdown of rhomboid domain containing 1 inhibits colorectal cancer cell growth

Authors:
- Junyi Han
- Junchao Bai
- Yao Yang
- Hua Yin
- Wei Gao
- Aiguo Lu
- Fei Liu
- Haiyan Ge
- Zhongmin Liu
- Jinyi Wang
- Lan Zhong
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Affiliations: Department of Gastroenterology, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China, Department of General Surgery, People's Hospital of Dayao, Yunnan 675400, P.R. China, Department of General Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China, Department of Thoracic Surgery, Shanghai East Hospital, Tongji University, Shanghai 200120, P.R. China
Published online on: February 17, 2015 https://doi.org/10.3892/mmr.2015.3365
Pages: 377-381

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Abstract

Rhomboid domain containing 1 (RHBDD1), is a member of the rhomboid protease family, which has a pivotal role in the progression of numerous severe malignancies. However, its role in colorectal carcinoma (CRC) remains to be elucidated. In the present study, RHBDD1 was shown to be widely expressed in CRC cell lines. Lentivirus‑mediated RNA interference was employed to knockdown RHBDD1 expression in RKO CRC cells. Functional analyses indicated that depletion of RHBDD1 expression resulted in significantly reduced CRC cell proliferation and colony formation, and induced a G0/G1 phase cell cycle arrest. The findings of the present study suggest that RHBDD1 may contribute to CRC tumorigenesis and serve as a potential therapeutic target in human CRC.

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1	Cancer Genome Atlas Network: Comprehensive molecular characterization of human colon and rectal cancer. Nature. 487:330–337. 2012. View Article : Google Scholar : PubMed/NCBI
2	Merika E, Saif MW, Katz A, Syrigos K and Morse M: Review. Colon cancer vaccines: an update. In Vivo. 24:607–628. 2010.PubMed/NCBI
3	Cunningham D, Atkin W, Lenz HJ, et al: Colorectal cancer. Lancet. 375:1030–1047. 2010. View Article : Google Scholar : PubMed/NCBI
4	Fearon ER: Molecular genetics of colorectal cancer. Annu Rev Pathol. 6:479–507. 2011. View Article : Google Scholar
5	Lemberg MK: Intramembrane proteolysis in regulated protein trafficking. Traffic. 12:1109–1118. 2011. View Article : Google Scholar : PubMed/NCBI
6	Brown MS, Ye J, Rawson RB and Goldstein JL: Regulated intramembrane proteolysis: a control mechanism conserved from bacteria to humans. Cell. 100:391–398. 2000. View Article : Google Scholar : PubMed/NCBI
7	Kroos L and Yu YT: Regulation of sigma factor activity during Bacillus subtilis development. Curr Opin Microbiol. 3:553–560. 2000. View Article : Google Scholar : PubMed/NCBI
8	Weihofen A and Martoglio B: Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 13:71–78. 2003. View Article : Google Scholar : PubMed/NCBI
9	Freeman M: Rhomboid proteases and their biological functions. Annu Rev Genet. 42:191–210. 2008. View Article : Google Scholar : PubMed/NCBI
10	Wolfe MS and Kopan R: Intramembrane proteolysis: theme and variations. Science. 305:1119–1123. 2004. View Article : Google Scholar : PubMed/NCBI
11	Urban S: Rhomboid proteins: conserved membrane proteases with divergent biological functions. Genes Dev. 20:3054–3068. 2006. View Article : Google Scholar : PubMed/NCBI
12	Koonin EV, Makarova KS, Rogozin IB, Davidovic L, Letellier MC and Pellegrini L: The rhomboids: a nearly ubiquitous family of intramembrane serine proteases that probably evolved by multiple ancient horizontal gene transfers. Genome Biol. 4:R192003. View Article : Google Scholar : PubMed/NCBI
13	Wasserman JD, Urban S and Freeman M: A family of rhomboid-like genes: Drosophila rhomboid-1 and roughoid/rhomboid-3 cooperate to activate EGF receptor signaling. Genes Dev. 14:1651–1663. 2000.PubMed/NCBI
14	McQuibban GA, Saurya S and Freeman M: Mitochondrial membrane remodelling regulated by a conserved rhomboid protease. Nature. 423:537–541. 2003. View Article : Google Scholar : PubMed/NCBI
15	Wang Y, Guan X, Fok KL, et al: A novel member of the Rhomboid family, RHBDD1, regulates BIK-mediated apoptosis. Cell Mol Life Sci. 65:3822–3829. 2008. View Article : Google Scholar : PubMed/NCBI
16	Lin YN, Gui FM, Shen H, et al: Expression of RHBDD1 gene in patients with chronic myeloid leukemia and its clinical significance. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 21:12–15. 2013.Chinese. PubMed/NCBI
17	Liu XN, Tang ZH, Zhang Y, et al: Lentivirus-mediated silencing of rhomboid domain containing 1 suppresses tumor growth and induces apoptosis in hepatoma HepG2 cells. Asian Pac J Cancer Prev. 14:5–9. 2013. View Article : Google Scholar : PubMed/NCBI
18	Urban S and Dickey SW: The rhomboid protease family: a decade of progress on function and mechanism. Genome Biol. 12:2312011. View Article : Google Scholar : PubMed/NCBI
19	Lee JR, Urban S, Garvey CF and Freeman M: Regulated intracellular ligand transport and proteolysis control EGF signal activation in Drosophila. Cell. 107:161–171. 2001. View Article : Google Scholar : PubMed/NCBI
20	Urban S, Lee JR and Freeman M: Drosophila rhomboid-1 defines a family of putative intramembrane serine proteases. Cell. 107:173–182. 2001. View Article : Google Scholar : PubMed/NCBI
21	Zou H, Thomas SM, Yan ZW, Grandis JR, Vogt A and Li LY: Human rhomboid family-1 gene RHBDF1 participates in GPCR-mediated transactivation of EGFR growth signals in head and neck squamous cancer cells. FASEB J. 23:425–432. 2009. View Article : Google Scholar :
22	Normanno N, Tejpar S, Morgillo F, De Luca A, Van Cutsem E and Ciardiello F: Implications for KRAS status and EGFR-targeted therapies in metastatic CRC. Nat Rev Clin Oncol. 6:519–527. 2009. View Article : Google Scholar : PubMed/NCBI
23	Wang Y, Song W, Li S, et al: GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules. BMC Cell Biol. 10:252009. View Article : Google Scholar : PubMed/NCBI