Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration

Zhao,Qing‑Mei; Kuang,Fei; Wu,Han; Zhang,Yu-Hao

doi:10.3892/mmr.2015.3418

Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration

Authors:
- Qing‑Mei Zhao
- Fei Kuang
- Han Wu
- Yu-Hao Zhang
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Affiliations: Department of Oncology, Sichuan Mianyang 404 Hospital, Mianyang, Sichuan 621000, P.R. China, Department of General Surgery, Changhai Hospital of The Second Military Medical University, Shanghai 200433, P.R. China, Department of General Surgery, The 1745th Hospital of the PLA, Zhangzhou, Fujian 562001, P.R. China
Published online on: March 4, 2015 https://doi.org/10.3892/mmr.2015.3418
Pages: 470-474

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Abstract

Colorectal cancer is one of the most commonly diagnosed types of cancer and is a leading cause of cancer‑associated mortality worldwide. Short chain enoyl coenzyme A hydratase 1 (ECHS1) is an important gene involved in the mitochondrial fatty acid β‑oxidation pathway. In addition, ECHS1 has been implicated in a variety of cancers, including breast, prostate, colon and liver cancer. The aim of the present study was to examine the expression of ECHS1 in the human HCT‑8 colorectal cancer cell line. The results showed that ECHS1 expression was significantly increased in poorly‑differentiated cells compared with that in well‑differentiated cells. In order to further investigate the functions of ECHS1 in colorectal cancer cells, a stably transfected HCT‑8 cell line expressing small interfering (si)RNA targeting the ECHS1 gene was established. The expression of the ECHS1 siRNA was found to reduce ECHS1 protein levels in ECHS1‑silenced cells by >40%. Cell proliferation and cell migration of the siECHS1 cells were characterized using Cell Counting Kit‑8 and Transwell assays, respectively, the results of which showed that the constitutive knockdown of the ECSH1 gene in HCT‑8 cells significantly inhibited cell proliferation and migration. Furthermore, decreased levels of Akt and glycogen synthase kinase (GSK)3β phosphorylation were observed in ECHS1‑silenced HCT‑8 cells compared with that of parental or pU6 empty vector‑transfected cells. In conclusion, the results of the present study suggested that ECHS1 may have an important role in colorectal cancer cell proliferation and migration via activation of Akt‑ and GSK3β‑associated signaling pathways.

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