Inhibitory effect of receptor for advanced glycation end product‑specific small interfering RNAs on the development of hepatic fibrosis in primary rat hepatic stellate cells

  • Authors:
    • Jin‑Rong Xia
    • Ting‑Ting Chen
    • Wei‑Dong Li
    • Feng‑Lin Lu
    • Juan Liu
    • Xiao‑Gang Cai
    • Qin Lu
    • Cui-Ping Yang
  • View Affiliations

  • Published online on: February 12, 2015     https://doi.org/10.3892/mmr.2015.3342
  • Pages: 569-574
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Abstract

Specific small interfering RNAs (siRNAs) targeting receptor for advanced glycation end products (RAGE) inhibit the expression of RAGE, α‑smooth muscle actin and type I collagen in the T6 hepatic stellate cells (HSCs), indicating that RAGE is important for the activation of HSCs and the expression of collagen. The present study aimed to investigate the effect of specific siRNAs targeting RAGE on the development of hepatic fibrosis (HF), using primary rat HSCs, which were isolated and cultured in vitro. The expression vectors for specific siRNAs targeting RAGE were constructed and transfected into primary rat HSCs. Untreated and nonspecific siRNA‑transfected primary rat HSCs served as controls. The expression levels of RAGE, interleukin‑6 (IL‑6), tumor necrosis factor‑α (TNF‑α), transforming growth factor‑β1 (TGF‑β1), connective tissue growth factor (CTGF), laminin (LN), hyaluronic acid (HA) and N‑terminal procollagen III propeptide (PIIINP) in primary HSCs were detected by reverse transcription quantitative polymerase chain reaction and western blotting. The mRNA and 42 kD protein expression of RAGE in the pAKD‑GR126‑transfected primary HSCs were significantly downregulated compared with those in the untreated and the pAKD‑negative control (NC)‑transfected controls. The mRNA and protein expression levels of IL‑6, TNF‑α, TGF‑β1, CTGF, LN, HA and PIIINP in the pAKD‑GR126‑transfected primary HSCs were also markedly downregulated compared with those in the untreated and pAKD‑NC‑transfected controls. Therefore, RAGE‑specific siRNAs inhibited the expression of RAGE in primary rat HSCs and inhibited the development of HF.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xia JR, Chen TT, Li WD, Lu FL, Liu J, Cai XG, Lu Q and Yang C: Inhibitory effect of receptor for advanced glycation end product‑specific small interfering RNAs on the development of hepatic fibrosis in primary rat hepatic stellate cells. Mol Med Rep 12: 569-574, 2015
APA
Xia, J., Chen, T., Li, W., Lu, F., Liu, J., Cai, X. ... Yang, C. (2015). Inhibitory effect of receptor for advanced glycation end product‑specific small interfering RNAs on the development of hepatic fibrosis in primary rat hepatic stellate cells. Molecular Medicine Reports, 12, 569-574. https://doi.org/10.3892/mmr.2015.3342
MLA
Xia, J., Chen, T., Li, W., Lu, F., Liu, J., Cai, X., Lu, Q., Yang, C."Inhibitory effect of receptor for advanced glycation end product‑specific small interfering RNAs on the development of hepatic fibrosis in primary rat hepatic stellate cells". Molecular Medicine Reports 12.1 (2015): 569-574.
Chicago
Xia, J., Chen, T., Li, W., Lu, F., Liu, J., Cai, X., Lu, Q., Yang, C."Inhibitory effect of receptor for advanced glycation end product‑specific small interfering RNAs on the development of hepatic fibrosis in primary rat hepatic stellate cells". Molecular Medicine Reports 12, no. 1 (2015): 569-574. https://doi.org/10.3892/mmr.2015.3342