Salicylate-induced changes in immediate-early genes in the hippocampal CA1 area

Wu,Hao; Xu,Feng‑Lei; Yin,Yong; Da,Peng; You,Xiao‑Dong; Xu,Hui‑Min; Tang,Yan

doi:10.3892/mmr.2015.3608

Salicylate-induced changes in immediate-early genes in the hippocampal CA1 area

Authors:
- Hao Wu
- Feng‑Lei Xu
- Yong Yin
- Peng Da
- Xiao‑Dong You
- Hui‑Min Xu
- Yan Tang
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Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, Nanjing General Hospital of Nanjing Military Area, Nanjing, Jiangsu 210002, P.R. China, Department of Otolaryngology, Changshu First Hospital, Suzhou, Jiangsu 215500, P.R. China
Published online on: April 14, 2015 https://doi.org/10.3892/mmr.2015.3608
Pages: 1625-1630
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Studies have suggested that salicylate affects neuronal function via interactions with specific membrane channels/receptors. However, the effect of salicylate on activity and synaptic morphology of the hippocampal Cornu Ammonis (CA) 1 area remains to be elucidated. The activation of immediate‑early genes (IEGs) was reported to correlate with neuronal activity, in particular activity‑regulated cytoskeleton‑associated protein and early growth response gene 1. The aim of the present study was to evaluate the expression of these IEGs, as well that of N‑methyl D‑aspartate (NMDA) receptor subunit 2B in rats following acute and chronic salicylate treatment. Protein and messenger RNA levels of all three genes were increased in rats following chronic administration of salicylate (300 mg/kg for 10 days), returning to baseline levels 14 days post‑cessation of treatment. The transient upregulation of gene expression following treatment was accompanied by ultrastructural alterations in hippocampal CA1 area synapses. An increase in synaptic interface curvature was observed as well as an increased number of presynaptic vesicles; in addition, postsynaptic densities thickened and lengthened. In conclusion, the results of the present study indicated that chronic exposure to salicylate may lead to structural alteration of hippocampal CA1 neurons, and it was suggested that this process occurs through induced expression of IEGs via NMDA receptor activation.

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