Feasibility and safety of porcine Descemet's membrane as a carrier for generating tissue-engineered corneal endothelium

  • Authors:
    • Yu‑Mei Diao
    • Jing Hong
  • View Affiliations

  • Published online on: April 23, 2015     https://doi.org/10.3892/mmr.2015.3665
  • Pages: 1929-1934
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Abstract

The aim of this study was to evaluate the feasibility and safety of porcine Descemet's membrane (DM) as a carrier for the generation of tissue‑engineered corneal endothelium by analyzing porcine endogenous retroviruses (PERVs) and the α‑gal epitope. The morphology of porcine and human DM was observed by hematoxylin and eosin staining and scanning electron microscopy. Immunohistochemical staining was used to investigate the location of α‑gal epitopes on porcine DM used for xenotransplantation. The porcine DM was treated with ethylene glycol diglycidyl ether (EDGE) for 2 weeks, and then the PERV gene sequences in porcine DM and DM‑EDGE were detected by polymerase chain reaction (PCR) and real‑time PCR, respectively. The porcine DM had tight basement membrane morphology, which was similar to human DM in terms of thickness. No positive immunohistochemical staining of the α‑gal epitope was detected in porcine DM. PERV expression of pol, gag, env‑A and env‑B was noted in porcine DM, but in DM‑EDGE it was completely degraded. Based on structural, immunological and etiological studies, porcine DM may be an ideal and viable carrier for the generation of tissue-engineered corneal endothelium.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Diao YM and Diao YM: Feasibility and safety of porcine Descemet's membrane as a carrier for generating tissue-engineered corneal endothelium. Mol Med Rep 12: 1929-1934, 2015
APA
Diao, Y., & Diao, Y. (2015). Feasibility and safety of porcine Descemet's membrane as a carrier for generating tissue-engineered corneal endothelium. Molecular Medicine Reports, 12, 1929-1934. https://doi.org/10.3892/mmr.2015.3665
MLA
Diao, Y., Hong, J."Feasibility and safety of porcine Descemet's membrane as a carrier for generating tissue-engineered corneal endothelium". Molecular Medicine Reports 12.2 (2015): 1929-1934.
Chicago
Diao, Y., Hong, J."Feasibility and safety of porcine Descemet's membrane as a carrier for generating tissue-engineered corneal endothelium". Molecular Medicine Reports 12, no. 2 (2015): 1929-1934. https://doi.org/10.3892/mmr.2015.3665