Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling

Gao,Peng; Yang,Jingxian; Gao,Xi; Xu,Dan; Niu,Dongge; Li,Jinglin; Wen,Qingping

doi:10.3892/mmr.2015.3632

Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling

Authors:
- Peng Gao
- Jingxian Yang
- Xi Gao
- Dan Xu
- Dongge Niu
- Jinglin Li
- Qingping Wen
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Affiliations: Department of Anesthesiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning 116600, P.R. China, Department of Anesthesiology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Published online on: April 16, 2015 https://doi.org/10.3892/mmr.2015.3632
Pages: 1971-1976

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Abstract

Acute lung injury (ALI) is among the most common causes of mortality in intensive care units. Previous studies have suggested that bone marrow‑derived mesenchymal stem cells (BMSCs) may attenuate pulmonary edema. In addition, alveolar epithelial cells type Ⅰ (ATⅠ) are involved in reducing the alveolar edema in response to ALI. However, the mechanism involved in improving the efficiency of differentiation of MSCs into ATⅠ remains to be elucidated. In the present study, the effect of salvianolic acid B (Sal B) on the differentiation of BMSCs into ATⅠ and the activities of the Wnt signaling pathways were investigated. The BMSCs were supplemented with conditioned medium (CM). The groups were as follows: i) CM group: BMSCs were supplemented with CM; ii) lithium chloride (LiCl) group: BMSCs were supplemented with CM and 5 mM LiCl; iii) Sal B group: BMSCs were supplemented with CM and 10 mM Sal B. The samples were collected and assessed on days 7 and 14. It was revealed that aquaporin (AQP)‑5 and T1α were expressed in BMSCs, and induction with LiCl or Sal B increased the expression of AQP‑5 and T1α. Furthermore, the Wnt‑1 and Wnt‑3a signaling pathways were activated during the differentiation of BMSCs into ATⅠ. In conclusion, it was suggested that the promotive effects of Sal B on the differentiation of BMSCs into ATⅠ occurred through the activation of Wnt signaling pathways.

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1	Rubenfeld GD, Caldwell E, Peabody E, et al: Incidence and outcomes of acute lung injury. N Engl J Med. 353:1685–1693. 2005. View Article : Google Scholar : PubMed/NCBI
2	Ortiz LA, Gambelli F, McBride C, et al: Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci USA. 100:8407–8411. 2003. View Article : Google Scholar : PubMed/NCBI
3	Rojas M, Xu J, Woods CR, et al: Bone marrow derived mesenchymal stem cells in repair of the injured lung. Am J Respir Cell Mol Biol. 33:145–152. 2005. View Article : Google Scholar : PubMed/NCBI
4	Kotton DN, Ma BY, Cardoso WV, Sanderson EA, Summer RS, et al: Bone marrow-derived cells as progenitors of lung alveolar epithelium. Development. 128:5181–5188. 2001.PubMed/NCBI
5	Zhuang P, Zhang Y, Cui G, Bian Y, Zhang M, Zhang J, Liu Y, Yang X, Isaiah AO, Lin Y, et al: Direct stimulation of adult neural stem/progenitor cells in vitro and neurogenesis in vivo by salvianolic acid B. PLoS One. 7:e356362012. View Article : Google Scholar : PubMed/NCBI
6	Guo G, Li B, Wang Y, Shan A, Shen W, Yuan L and Zhong S: Effects of salvianolic acid B on proliferation, neurite outgrowth and differentiation of neural stem cells derived from the cerebral cortex of embryonic mice. Sci China Life Sci. 53:653–662. 2010. View Article : Google Scholar : PubMed/NCBI
7	Chan SS, Chen JH, Hwang SM, Wang IJ, Li HJ, Lee RT and Hsieh PC: Salvianolic acid B-vitamin C synergy in cardiac differentiation from embryonic stem cells. Biochem Biophys Res Commun. 387:723–728. 2009. View Article : Google Scholar : PubMed/NCBI
8	Wang Y, Sun Z, Qiu X, Li Y, Qin J and Han X: Roles of Wnt/beta-catenin signaling in epithelial differentiation of mesenchymal stem cells. Biochem Biophys Res Commun. 390:1309–1314. 2009. View Article : Google Scholar : PubMed/NCBI
9	Lee H, Bae S, Choi BW and Yoon Y: WNT/β-catenin pathway is modulated in asthma patients and LPS-stimulated RAW264.7 macrophage cell line. Immunopharmacol Immunotoxicol. 34:56–65. 2012. View Article : Google Scholar
10	Baarsma HA, Spanjer AI, Haitsma G, et al: Activation of WNT/β-catenin signaling in pulmonary fibroblasts by TGF-β₁ is increased in chronic obstructive pulmonary disease. PLoS One. 6:e254502011. View Article : Google Scholar
11	Ionescu L, Byrne RN, van Haaften T, et al: Stem cell conditioned medium improves acute lung injury in mice: in vivo evidence for stem cell paracrine action. Am J Physiol Lung Cell Mol Physiol. 303:L967–L977. 2012. View Article : Google Scholar : PubMed/NCBI
12	Parambil JG, Myers JL, Aubry MC and Ryu JH: Causes and prognosis of diffuse alveolar damage diagnosed on surgical lung biopsy. Chest. 132:50–57. 2007. View Article : Google Scholar : PubMed/NCBI
13	Gao C, Li R, Huan J, et al: Caveolin-1 siRNA increases the pulmonary microvascular and alveolar epithelial permeability in rats. J Trauma. 70:210–219. 2011. View Article : Google Scholar
14	Lu B, Ye Z, Deng Y, Wu H and Feng J: MEK/ERK pathway mediates cytoprotection of salvianolic acid B against oxidative stress-induced apoptosis in rat bone marrow stem cells. Cell Biol Int. 34:1063–1068. 2010. View Article : Google Scholar : PubMed/NCBI
15	Lay IS, Hsieh CC, Chiu JH, Shiao MS, Lui WY and Wu CW: Salvianolic acid B enhances in vitro angiogenesis and improves skin flap survival in Sprague-Dawley rats. J Surg Res. 115:279–285. 2003. View Article : Google Scholar : PubMed/NCBI
16	Chen YL, Hu CS, Lin FY, et al: Salvianolic acid B attenuates cyclooxygenase-2 expression in vitro in LPS-treated human aortic smooth muscle cells and in vivo in the apolipoprotein-E-deficient mouse aorta. J Cell Biochem. 98:618–631. 2006. View Article : Google Scholar : PubMed/NCBI
17	Lin F, Liu YY, Xu B, et al: Salvianolic acid B protects from pulmonary microcirculation disturbance induced by lipopolysaccharide in rat. Shock. 39:317–325. 2013. View Article : Google Scholar : PubMed/NCBI
18	Logan CY and Nusse R: The Wnt signaling pathway in development and disease. Annu Rev Cell Dev Biol. 20:781–810. 2004. View Article : Google Scholar : PubMed/NCBI
19	Gordon MD and Nusse R: Wnt signaling: Multiple pathways, multiple receptors, and multiple transcription factors. J Biol Chem. 281:22429–22433. 2006. View Article : Google Scholar : PubMed/NCBI
20	MacDonald BT, Tamai K and He X: Wnt/beta-catenin signaling: Components, mechanisms and diseases. Dev Cell. 17:9–26. 2009. View Article : Google Scholar : PubMed/NCBI