Pulsed electromagnetic wave exposure induces ultrastructural damage and upregulated expression of heat shock protein 70 in the rat adenohypophysis

Cheng,Kang; Ren,Dong‑Qing; Yi,Jun; Zhou,Xiao‑Guang; Yang,Wen‑Qing; Chen,Yong‑Bin; Li,Yong‑Qiang; Huang,Xiao‑Feng; Zeng,Gui‑Ying

doi:10.3892/mmr.2015.3627

Pulsed electromagnetic wave exposure induces ultrastructural damage and upregulated expression of heat shock protein 70 in the rat adenohypophysis

Authors:
- Kang Cheng
- Dong‑Qing Ren
- Jun Yi
- Xiao‑Guang Zhou
- Wen‑Qing Yang
- Yong‑Bin Chen
- Yong‑Qiang Li
- Xiao‑Feng Huang
- Gui‑Ying Zeng
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Affiliations: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Radiation Medicine and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Urology, Bayi Children's Hospital Affiliated to People's Liberation Army General Hospital, Beijing 100700, P.R. China, Lintong Sanatorium of Chinese PLA Lanzhou Command, Xi'an, Shaanxi 710600, P.R. China, Electron Microscopy Center, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
Published online on: April 16, 2015 https://doi.org/10.3892/mmr.2015.3627
Pages: 2175-2180

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Abstract

The aim of the present study was to investigate the ultrastructural damage and the expression of heat shock protein 70 (HSP70) in the rat adenohypophysis following pulsed electromagnetic wave (PEMW) exposure. The rats were randomly divided into four groups: Sham PEMW exposure, 1x104 pulses of PEMW exposure, 1x105 pulses of PEMW exposure and 3x105 pulses of PEMW exposure. Whole body radiation of 1x104 pulses, 1x105 pulses and 3x105 pulses of PEMW were delivered with a field strength of 100 kV/m. The rats in each group (n=6 in each) were sacrificed 12, 24, 48 and 96 h after PEMW exposure. Transmission electron microscopy was then used to detect the ultrastructural changes and immunocytochemistry was used to examine the expression of HSP70. Cellular damage, including mitochondrial vacuolation occurred as early as 12 h after PEMW exposure.More severe cellular damages, including cell degeneration and necrosis, occurred 24 and 48 h after PEMW exposure. The PEMW‑induced cellular damage increased as the number of PEMW pulses increased. In addition, the expression of HSP70 significantly increased following PEMW exposure and peaked after 12 h. These findings suggested that PEMW induced ultrastructural damages in the rat adenohypophysis and that HSP70 may have contributed to the PEMW-induced adenohypophyseal damage.

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