Identification of lymphatic metastasis‑associated genes in a metastatic ovarian cancer cell line

  • Authors:
    • Yu Kang
    • Tao Pu
    • Qingqing Cai
    • Shanshan Hong
    • Mingxing Zhang
    • Guiling Li
    • Zhiling Zhu
    • Congjian Xu
  • View Affiliations

  • Published online on: May 7, 2015     https://doi.org/10.3892/mmr.2015.3743
  • Pages: 2741-2748
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Abstract

Ovarian cancer (OC) is the gynecological malignancy with the highest rate of mortality, and lymphatic metastasis is a critical factor in disease recurrence and prognosis. In the current study, the SKOV‑3/LN403 cell line, which has high potential for lymph node metastasis was established as a result of four rounds of selection from retroperitoneal lymph nodes following intraperitoneal injections of SKOV‑3 ovarian adenocarcinoma cells. In comparison to the parental SKOV‑3 cell line, SKOV‑3/LN403 has a higher rate of proliferation, is more invasive and exhibits greater resistance to paclitaxel. Subsequently, a novel animal model of OC lymphatic metastasis was developed with SKOV‑3/LN403 cells and a high incidence of positive metastatic lymph nodes, peritoneal dissemination and bloody ascites were observed, which mimicked the clinical outcome of patients with OC. Analysis of the gene expression profiles of SKOV‑3 and SKOV‑3/LN403 cells identified several genes and pathways that may be involved in lymphatic metastasis of OC. The induction of focal adhesion kinase expression provides a potential therapeutic target for OC lymphatic metastasis.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kang Y, Pu T, Cai Q, Hong S, Zhang M, Li G, Zhu Z and Xu C: Identification of lymphatic metastasis‑associated genes in a metastatic ovarian cancer cell line. Mol Med Rep 12: 2741-2748, 2015
APA
Kang, Y., Pu, T., Cai, Q., Hong, S., Zhang, M., Li, G. ... Xu, C. (2015). Identification of lymphatic metastasis‑associated genes in a metastatic ovarian cancer cell line. Molecular Medicine Reports, 12, 2741-2748. https://doi.org/10.3892/mmr.2015.3743
MLA
Kang, Y., Pu, T., Cai, Q., Hong, S., Zhang, M., Li, G., Zhu, Z., Xu, C."Identification of lymphatic metastasis‑associated genes in a metastatic ovarian cancer cell line". Molecular Medicine Reports 12.2 (2015): 2741-2748.
Chicago
Kang, Y., Pu, T., Cai, Q., Hong, S., Zhang, M., Li, G., Zhu, Z., Xu, C."Identification of lymphatic metastasis‑associated genes in a metastatic ovarian cancer cell line". Molecular Medicine Reports 12, no. 2 (2015): 2741-2748. https://doi.org/10.3892/mmr.2015.3743