Expression profile of plasma microRNAs in premature infants with respiratory distress syndrome

Kan,Qing; Ding,Sufang; Yang,Yang; Zhou,Xiaoyu

doi:10.3892/mmr.2015.3699

Expression profile of plasma microRNAs in premature infants with respiratory distress syndrome

Authors:
- Qing Kan
- Sufang Ding
- Yang Yang
- Xiaoyu Zhou
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Affiliations: Department of Neonatology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, Department of Neonatology, Huaian Maternity and Child Health Care Hospital, Huaian, Jiangsu 223002, P.R. China
Published online on: April 29, 2015 https://doi.org/10.3892/mmr.2015.3699
Pages: 2858-2864

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Abstract

As well-known regulators of gene expression, microRNAs (miRNAs) are important not only in cell proliferation and differentiation, but also in tumorigenesis and organ development. It has been estimated that miRNAs may be responsible for regulating the expression of almost one third of the human genome. Simultaneously, with advances in neonatal care in the clinic, an increased number of premature infants are being saved and, thus, respiratory distress syndrome (RDS) has become more common. However, previous non‑miRNA studies have suggested their connection with RDS. In the present study, a miRNA microarray, including >1,891 capture probes was used to compared the expression profiles of plasma miRNAs between RDS and control groups. miRNAs, which were observed to have consistent fold‑changes (fold‑change ≥1.3) between the two groups were selected and validated using reverse transcription‑quantitative polymerase chain reaction. As a result, 171 differentially expressed miRNAs were identified, including two upregulated and seven downregulated miRNAs. Of these miRNAs, four were selected as having higher fold‑changes between the two groups. This is the first time, to the best ouf our knowledge, that these nine miRNAs have been reported in RDS. It was hypothesized that these novel miRNAs may be important in RDS, and may provide meaningful biomarkers for the diagnosis of RDS.

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1	Sweet D, Carnielli V, Greisen G, et al: European consensus guidelines on the management of neonatal respiratory distress syndrome in preterm infants - 2013 update. Neonatology. 103:353–368. 2013. View Article : Google Scholar
2	Soll RF: Early versus delayed selective surfactant treatment for neonatal respiratory distress syndrome. Neonatology. 104:124–126. 2013. View Article : Google Scholar
3	Chang LW and Li WB: Fetal and neonatal lung development. J Appl Clin Pediatrics. 26:1065–1067. 2011.
4	Morrisey EE: Wnt signaling and pulmonary fibrosis. Am J Pathol. 162:1393–1397. 2003. View Article : Google Scholar : PubMed/NCBI
5	Chen XM, Splinter PL, O’Hara SP, et al: A cellular micro-RNA, let-7i, regulates toll-like receptor 4 expression and contributes to cholangiocyte immune responses against cryptosporidium parvum infection. J Biol Chem. 282:28929–28938. 2007. View Article : Google Scholar : PubMed/NCBI
6	Johnson CD, Esquela-Kerscher A, Stefani G, et al: The let-7 microRNA represses cell proliferation pathways in human cells. Cancer Res. 67:7713–7722. 2007. View Article : Google Scholar : PubMed/NCBI
7	Lu Y, Thomson JM, Wong HY, et al: Transgenic overexpression of the microRNA miR-17-92 cluster promotes proliferation and inhibits differentiation of lung epithelial progenitor cells. Dev Biol. 310:442–453. 2007. View Article : Google Scholar : PubMed/NCBI
8	Ramasamy SK, Mailleux AA, Gupte VV, et al: Fgf10 dosage is critical for the amplification of epithelial cell progenitors and for the formation of multiple mesenchymal lineages during lung development. Dev Biol. 307:237–247. 2007. View Article : Google Scholar : PubMed/NCBI
9	Sayed D and Abdellatif M: MicroRNAs in development and disease. Physiol Rev. 91:827–887. 2011. View Article : Google Scholar : PubMed/NCBI
10	Lim LP, Lau NC, Garrett-Engele P, et al: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature. 433:769–773. 2005. View Article : Google Scholar : PubMed/NCBI
11	Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI
12	Taylor DD and Gercel-Taylor C: MicroRNA signatures of tumor derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol. 110:13–21. 2008. View Article : Google Scholar : PubMed/NCBI
13	Hiroki E, Akahira J, Suzuki F, et al: Changes in microRNA expression levels correlate with clinicopathological features and prognoses in endometrial serous adenocarci nomas. Cancer Sci. 101:241–249. 2010. View Article : Google Scholar
14	Grosshans H and Filipowicz W: Molecular biology: the expanding world of small RNAs. Nature. 451:414–416. 2008. View Article : Google Scholar : PubMed/NCBI
15	Mandel EE; Paris Da: The routine use of the serum flocculation reaction with Hayem’s solution. J Lab Clin Med. 33:16291948.
16	Chen X, Ba Y, Ma L, et al: Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 18:997–1006. 2008. View Article : Google Scholar : PubMed/NCBI
17	El-hefnawy T, Raja S, Kelly L, et al: Characterization of amplifiable, Circulating RNA in plasma and its potential as a tool for cancer diagnostics. Clin Chem. 50:564–573. 2004. View Article : Google Scholar : PubMed/NCBI
18	György B, Szabó TG, Pásztói M, Pál Z, Misják P, Aradi B, László V, Pállinger E, Pap E, Kittel A, Nagy G, Falus A and Buzás EI: Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles. Cell Mol Life Sci. 68:2667–2688. 2011. View Article : Google Scholar : PubMed/NCBI
19	Ai J, Zhang R, Li Y, et al: Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarction. Biochem Biophys Res Commun. 391:73–77. 2010. View Article : Google Scholar
20	Yuan L and Chen C: Prevention and treatment of neonatal respiratory distress syndrome in Europe guide. Chin J Pediatr. 49:27–33. 2011.
21	World Medical Association: World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects. JAMA. 310:2191–2194. 2013. View Article : Google Scholar : PubMed/NCBI
22	Jobe AH: The new bronchopulmonary dysplasia. Curr Opin Pediatr. 23:167–172. 2011. View Article : Google Scholar
23	Ruegger C, Hegglin M, Adams M, et al: Population based trends in mortality, morbidity and treatment for very preterm-and very low birth weight infants over 12 years. BMC Pediatr. 12:172012. View Article : Google Scholar
24	Ventura A, Young AG, Winslow MM, et al: Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Cell. 132:875–886. 2008. View Article : Google Scholar : PubMed/NCBI
25	Zhang X, Zhu J, Xing R, et al: miR-513a-3p sensitizes human lung adenocarcinoma cells to chemotherapy by targeting GSTP1. Lung Cancer. 77:488–494. 2012. View Article : Google Scholar : PubMed/NCBI
26	Yang Y, Kai G, Pu XD, et al: Expression profile of microRNAs in fetal lung development of Sprague-Dawley rats. Int J Mol Med. 29:393–402. 2012.
27	Kulshreshtha R, Ferracin M, Wojcik SE, et al: A microRNA signature of hypoxia. Mol Cell Biol. 27:1859–1867. 2007. View Article : Google Scholar :
28	Huang G, Mills L and Worth LL: Expression of human glutathione S-transferase P1 mediates the chemosensitivity of osteosarcoma cells. Mol Cancer Ther. 6:1610–1619. 2007. View Article : Google Scholar : PubMed/NCBI
29	Ishii T, Fujishiro M, Masuda M, et al: A methylated oligonucleotide induced methylation of GSTP1 promoter and suppressed its expression in A549 lung adenocarcinoma cells. Cancer Lett. 212:211–223. 2004. View Article : Google Scholar : PubMed/NCBI
30	Wilfred BR, Wang WX and Nelson PT: Energizing miRNA research: a review of the role of miRNAs in lipid metabolism, with a prediction that miR-103/107 regulates human metabolic pathways. Mol Genet Metab. 91:209–217. 2007. View Article : Google Scholar : PubMed/NCBI
31	Ellis KL, Cameron VA, Troughton RW, Frampton CM, et al: Circulating microRNAs as candidate markers to distinguish heart failure in breathless patients. Eur J Heart Fail. 15:1138–1147. 2013. View Article : Google Scholar : PubMed/NCBI
32	Lu Z, Li Y, Takwi A, et al: miR-301a as an NF- kB activator in pancreatic cancer cells. EMBO J. 30:57–67. 2011. View Article : Google Scholar :
33	Kotecha S: cytokine in chronic lung disease of prematurity. Eur J Pediatr. 155(suppl 2): 14–17. 1996. View Article : Google Scholar
34	Munshi UK, Niu JO, Siddiq MM, et al: Elevation of interleukin-8 and interleukin-6 precedes the influx of neutrophils in tracheal aspirates from preterm infants who develop bronchopulmonary dysplasia. Pediatr Pulmonol. 24:331–336. 1997. View Article : Google Scholar
35	Takahashi N, Nakaoka T and Yamashita N: Profiling of immune-related microRNA expression in human cord blood and adult peripheral blood cells upon proinflammatory stimulation. Eur J Haematol. 88:31–38. 2012. View Article : Google Scholar