MicroRNA‑29a inhibits cell migration and invasion by targeting Roundabout 1 in breast cancer cells Retraction in /10.3892/mmr.2023.13151

Li,Hui; Luo,Jiashun; Xu,Bin; Luo,Kaijun; Hou,Juan

doi:10.3892/mmr.2015.3749

MicroRNA‑29a inhibits cell migration and invasion by targeting Roundabout 1 in breast cancer cells

Retraction in: /10.3892/mmr.2023.13151

Authors:
- Hui Li
- Jiashun Luo
- Bin Xu
- Kaijun Luo
- Juan Hou
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Affiliations: Department of Microbiology and Immunology, Medical School of Jishou University, Jishou, Hunan 416000, P.R. China, Institute of Medical Research, Medical School of Jishou University, Jishou, Hunan 416000, P.R. China
Published online on: May 7, 2015 https://doi.org/10.3892/mmr.2015.3749
Pages: 3121-3126

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Abstract

MicroRNAs (miRs) are a group of small non‑coding RNAs, which influence the development and progression of cancer by mediating the protein expression levels of tumor suppressors or oncogenes. miR‑29a has been reported to be involved in the pathogenesis of breast cancer. However, its precise functions, and the mechanisms underlying these effects, remain to be elucidated. The present study demonstrated that miR‑29a was markedly downregulated in breast cancer tissues and cell lines. The protein expression of Roundabout 1 (Robo1) was negatively regulated by miR‑29a in MCF‑7 breast cancer cells. Luciferase reporter assays identified Robo1 as a direct target of miR‑29a in MCF‑7 cells. Additionally, upregulation of miR‑29a inhibited MCF‑7 cell migration and invasion, which was attenuated by the restoration of the expression of Robo1. Finally, it was shown that the expression of Robo1 mRNA and protein was increased in breast cancer tissues and cell lines. Therefore, it was hypothesized that miR‑29a inhibits cell migration and invasion in breast cancer cells, at least in part, by directly targeting Robo1. In conclusion, the present study highlights the importance of miR‑29a and Robo1 in the progression of breast cancer.

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