Therapeutic effects of Lactobacillus casei Qian treatment in activated carbon‑induced constipated mice

Zhao,Xin; Suo,Hua‑Yi; Qian,Yu; Li,Gui‑Jie; Liu,Zhen‑Hu; Li,Jian

doi:10.3892/mmr.2015.3737

Therapeutic effects of Lactobacillus casei Qian treatment in activated carbon‑induced constipated mice

Authors:
- Xin Zhao
- Hua‑Yi Suo
- Yu Qian
- Gui‑Jie Li
- Zhen‑Hu Liu
- Jian Li
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Affiliations: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, College of Food Science, Southwest University, Chongqing 400715, P.R. China, Science and Technology Administration, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China, Institute of Qinghai‑Tibetan Plateau, Southwest University for Nationalities, Chengdu, Sichuan 610041, P.R. China
Published online on: May 6, 2015 https://doi.org/10.3892/mmr.2015.3737
Pages: 3191-3199

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Abstract

In the present study, the therapeutic effects of Lactobacillus casei Qian (LC‑Qian), the key microorganism in Tibetan yak yoghurt, on activated carbon‑induced constipation were determined in vivo. ICR mice were treated with LC‑Qian for nine days by oral administration. The body weight, defecation status, gastrointestinal transit and defecation time of mice were assessed, and the serum levels of motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP) and vasoactive intestinal peptide (VIP) were further evaluated. Bisacodyl was used as the positive control. The time until the first black stool defecation following carbon intake of the normal, control, 100 mg/kg bisacodyl‑treated, Lactobacillus bulgaricus (LB)‑treated, LC‑Qian (L)‑ and LC‑Qian (H)‑treated mice was 93, 231, 121, 194, 172 and 157 min, respectively. Following treatment with LC‑Qian, the gastrointestinal transit was increased to 52.4% [LC‑Qian (L)] and 65.8% [LC‑Qian (H)], while that in the group treated with the common lactic acid bacteria of LB was 40.3%. The MTL, Gas, ET, AChE, SP and VIP serum levels were significantly increased and levels of SS were reduced in mice following LC‑Qian treatment compared with those in the control mice (P<0.05). Reverse transcription quantitative polymerase chain reaction indicated that LC‑Qian raised the c‑Kit, GDNF as well as SCF mRNA expression levels and reduced the TRPV1 and NOS expression levels in tissue of the small intestine in mice. These results suggested that lactic acid bacteria prevent constipation in mice, among which LC‑Qian was the most effective.

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