Gender-specific association between the cytoplasmic poly(A) binding protein 4 rs4660293 single nucleotide polymorphism and serum lipid levels

Wu,Jian; Yin,Rui-Xing; Guo,Tao; Lin,Quan-Zhen; Shen,Shao-Wen; Sun,Jia-Qi; Shi,Guang-Yuan; Wu,Jin-Zhen; Yang,De-Zhai; Lin,Wei-Xiong

doi:10.3892/mmr.2015.3823

Gender-specific association between the cytoplasmic poly(A) binding protein 4 rs4660293 single nucleotide polymorphism and serum lipid levels

Authors:
- Jian Wu
- Rui-Xing Yin
- Tao Guo
- Quan-Zhen Lin
- Shao-Wen Shen
- Jia-Qi Sun
- Guang-Yuan Shi
- Jin-Zhen Wu
- De-Zhai Yang
- Wei-Xiong Lin
View Affiliations

Affiliations: Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Molecular Biology, Medical Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Published online on: May 22, 2015 https://doi.org/10.3892/mmr.2015.3823
Pages: 3476-3486
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Cytoplasmic poly(A) binding protein 4 (PABPC4) is an RNA-processing protein which has an important role in regulating gene expression. The association of the PABPC4 rs4660293 single nucleotide polymorphism (SNP) and serum lipid profiles has, to the best of our knowledge, not previously been studied in the Chinese population. The present study aimed to investigate the association between the PABPC4 rs4660293 SNP and several environmental factors with serum lipid levels in the Mulao and Han populations. A total of 727 individuals of Mulao nationality and 729 individuals of Han nationality were randomly selected from stratified randomized samples from a previous study by our group. Genotypes of the PABPC4 rs4660293 SNP were determined via polymerase chain reaction and restriction fragment length polymorphism analyses and subsequently confirmed by direct sequencing. Serum levels of low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) B were higher in the Mulao group than those in the Han group (P<0.01 for each). The genotypic and allelic frequencies of the PABPC4 rs4660293 SNP were significantly different between males and females in the Mulao population (P<0.05 for each), while no significant difference was detected between those of males and females amongst the Han population. The frequency of the G allele was higher in Mulao males than in Mulao females (22.12 vs. 13.44%). The G allele carriers were found to have higher total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and ApoAI levels in Han females but not in Han males, and lower TC and HDL-C levels in Mulao females but not in Mulao males than those of the G allele non-carriers (P<0.05 for all). These associations were confirmed by multiple linear regression analysis (P<0.05‑0.001). Serum lipid parameters were also correlated with multiple environmental factors (P<0.05‑0.001). The PABPC4 rs4660293 SNP was associated with serum TC, HDL-C, LDL-C and ApoAI levels in these study populations; however, the association varied between the Mulao and Han populations. A gender-specific association was identified in the populations of the two ethnic groups.

View Figures

View References

1	Roberts R and Stewart AF: Genes and coronary artery disease: where are we? J Am Coll Cardiol. 60:1715–1721. 2012. View Article : Google Scholar : PubMed/NCBI
2	Satoh H, Nishino T, Tomita K and Tsutsui H: Fasting triglyceride is a significant risk factor for coronary artery disease in middle-aged Japanese men. Circ J. 70:227–231. 2006. View Article : Google Scholar : PubMed/NCBI
3	Arai H, Yamamoto A, Matsuzawa Y, et al: Serum lipid survey and its recent trend in the general Japanese population in 2000. J Atheroscler Thromb. 12:98–106. 2005. View Article : Google Scholar : PubMed/NCBI
4	Hokanson JE and Austin MA: Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: a meta-analysis of population-based prospective studies. J Cardiovasc Risk. 3:213–219. 1996. View Article : Google Scholar : PubMed/NCBI
5	Austin MA: Plasma triglyceride as a risk factor for coronary heart disease. The epidemiologic evidence and beyond. Am J Epidemiol. 129:249–259. 1989.PubMed/NCBI
6	World Health Organization: The top 10 causes of death: Media centre fact sheets. 2014, http://www.who.int/mediacentre/fact-sheets/fs310/en. Accessed March 14 2015.
7	Murray CJ and Lopez AD: Alternative projections of mortality and disability by cause 1990–2020: Global Burden of Disease Study. Lancet. 349:1498–1504. 1997. View Article : Google Scholar : PubMed/NCBI
8	Burnett JR: Lipids, lipoproteins, atherosclerosis and cardiovascular disease. Clin Biochem Rev. 25:22004.
9	Jackson R, Lawes CM, Bennett DA, Milne RJ and Rodgers A: Treatment with drugs to lower blood pressure and blood cholesterol based on an individual's absolute cardiovascular risk. Lancet. 365:434–441. 2005. View Article : Google Scholar : PubMed/NCBI
10	Sonntag F, Schaefer JR, Gitt AK, et al: Lipid therapy in daily routine. Dtsch Med Wochenschr. 137:2047–2052. 2012.In German. PubMed/NCBI
11	Heller DA, de Faire U, Pedersen NL, Dahlén G and McClearn GE: Genetic and environmental influences on serum lipid levels in twins. N Engl J Med. 328:1150–1156. 1993. View Article : Google Scholar : PubMed/NCBI
12	Pollin TI, Hsueh WC, Steinle NI, et al: A genome-wide scan of serum lipid levels in the Old Order Amish. Atherosclerosis. 173:89–96. 2004. View Article : Google Scholar : PubMed/NCBI
13	Pilia G, Chen WM, Scuteri A, et al: Heritability of cardiovascular and personality traits in 6,148 Sardinians. PLoS Genet. 2:e1322006. View Article : Google Scholar : PubMed/NCBI
14	Dumitrescu L, Carty CL, Taylor K, et al: Genetic determinants of lipid traits in diverse populations from the population architecture using genomics and epidemiology (PAGE) study. PLoS Genet. 7:e10021382011. View Article : Google Scholar : PubMed/NCBI
15	Hegele RA: Plasma lipoproteins: genetic influences and clinical implications. Nat Rev Genet. 10:109–121. 2009. View Article : Google Scholar : PubMed/NCBI
16	Kathiresan S, Manning AK, Demissie S, et al: A genome-wide association study for blood lipid phenotypes in the Framingham Heart Study. BMC Med Genet. 8(Suppl 1): S172007. View Article : Google Scholar : PubMed/NCBI
17	Teslovich TM, Musunuru K, Smith AV, et al: Biological, clinical and population relevance of 95 loci for blood lipids. Nature. 466:707–713. 2010. View Article : Google Scholar : PubMed/NCBI
18	Dehghan A, Dupuis J, Barbalic M, et al: Meta-analysis of genome-wide association studies in >80,000 subjects identifies multiple loci for C-reactive protein levels. Circulation. 123:731–738. 2011. View Article : Google Scholar : PubMed/NCBI
19	Danesh J, Wheeler JG, Hirschfield GM, et al: C-reactive protein and other circulating markers of inflammation in the prediction of coronary heart disease. N Engl J Med. 350:1387–1397. 2004. View Article : Google Scholar : PubMed/NCBI
20	Middelberg RP, Heath AC, Madden PA, et al: Evidence of differential allelic effects between adolescents and adults for plasma high-density lipoprotein. PLoS One. 7:e356052012. View Article : Google Scholar : PubMed/NCBI
21	Gorgoni B, Richardson WA, Burgess HM, et al: Poly(A)-binding proteins are functionally distinct and have essential roles during vertebrate development. Proc Natl Acad Sci USA. 108:7844–7849. 2011. View Article : Google Scholar : PubMed/NCBI
22	Villalba A, Coll O and Gebauer F: Cytoplasmic polyadenylation and translational control. Curr Opin Genet Dev. 21:452–457. 2011. View Article : Google Scholar : PubMed/NCBI
23	http://www.stats.gov.cn/tjsj/pcsj/rkpc/5rp/index.htm (In Chinese) Accessed August 16, 2013.
24	Yan TT, Yin RX, Li Q, et al: Sex-specific association of rs16996148 SNP in the NCAN/CILP2/PBX4 and serum lipid levels in the Mulao and Han populations. Lipids Health Dis. 10:2482011. View Article : Google Scholar
25	Xu L, Deng QY, Li SF, et al: Genetic analysis of Mulao nationality using 15 short tandem repeats. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 25:96–100. 2008.In Chinese. PubMed/NCBI
26	Li Q, Yin RX, Wei XL, et al: ATP-binding cassette transporter G5 and G8 polymorphisms and several environmental factors with serum lipid levels. PLoS One. 7:e379722012. View Article : Google Scholar : PubMed/NCBI
27	An epidemiological study of cardiovascular and cardiopulmonary disease risk factors in four populations in the People's Republic of China. Baseline report from the P.R.C.-U.S.A. Collaborative Study. People's Republic of China - United States Cardiovascular and Cardiopulmonary Epidemiology Research Group. Circulation. 85:1083–1096. 1992. View Article : Google Scholar
28	Ruixing Y, Weixiong L, Hanjun Y, et al: Diet, lifestyle, and blood pressure of the middle-aged and elderly in the Guangxi Bai Ku Yao and Han populations. Am J Hypertens. 21:382–387. 2008. View Article : Google Scholar : PubMed/NCBI
29	Dian-bao Long: The food custom of Mulao. Journal of Hechi University. 25:99–101. 2005.In Chinese.
30	Yuan-fu Lan: Ethnography of marriage customary law of the Mulao ethnic group in Luocheng. Journal of Guangxi University For Nationalities. 31:99–102. 2009.In Chinese.
31	Craig WY, Palomaki GE and Haddow JE: Cigarette smoking and serum lipid and lipoprotein concentrations: an analysis of published data. BMJ. 298:784–788. 1989. View Article : Google Scholar : PubMed/NCBI
32	Brinton EA: Effects of ethanol intake on lipoproteins and atherosclerosis. Curr Opin Lipidol. 21:346–351. 2010. View Article : Google Scholar : PubMed/NCBI
33	Onat A, Hergenc G, Dursunoglu D, et al: Associations of alcohol consumption with blood pressure, lipoproteins, and subclinical inflammation among Turks. Alcohol. 42:593–601. 2008. View Article : Google Scholar : PubMed/NCBI