Hydrogen sulfide attenuates doxorubicin‑induced cardiotoxicity by inhibiting calreticulin expression in H9c2 cells

  • Authors:
    • Mi‑Hua Liu
    • Yuan Zhang
    • Xiao‑Long Lin
    • Jun He
    • Tian‑Ping Tan
    • Shao‑Jian Wu
    • Shan Yu
    • Li Chen
    • Yu‑Dan Chen
    • Hong‑Yun Fu
    • Cong Yuan
    • Jian Li
  • View Affiliations

  • Published online on: July 2, 2015     https://doi.org/10.3892/mmr.2015.4020
  • Pages: 5197-5202
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Abstract

Doxorubicin (DOX) is a potent and currently available antitumor therapeutic agent; however, its clinical application is limited by the occurrence of cardiotoxicity. Preliminary evidence indicates that hydrogen sulfide (H2S) may exert protective effects against DOX cardiotoxicity. Therefore, the aim of the present study was to investigate whether calreticulin (CRT) is involved in the cardioprotection of H2S against DOX‑induced cardiotoxicity. DOX was observed to markedly induce injuries, including cytotoxicity and apoptosis, and also enhance the expression level of CRT. Notably, pretreatment of H9c2 cells with sodium hydrosulfide (a donor of H2S) significantly attenuated the decreased cell viability, the increased apoptosis rate and the increased expression level of CRT in H9c2 cells. In addition, pretreatment of H9c2 cells with N‑acetyl‑L‑cysteine, a scavenger of reactive oxygen species (ROS) prior to exposure to DOX, markedly decreased the expression of CRT. These results indicate that exogenous H2S attenuates DOX‑induced cardiotoxicity by inhibiting CRT expression in H9c2 cardiac cells.
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October-2015
Volume 12 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu MH, Zhang Y, Lin XL, He J, Tan TP, Wu SJ, Yu S, Chen L, Chen YD, Fu HY, Fu HY, et al: Hydrogen sulfide attenuates doxorubicin‑induced cardiotoxicity by inhibiting calreticulin expression in H9c2 cells. Mol Med Rep 12: 5197-5202, 2015.
APA
Liu, M., Zhang, Y., Lin, X., He, J., Tan, T., Wu, S. ... Li, J. (2015). Hydrogen sulfide attenuates doxorubicin‑induced cardiotoxicity by inhibiting calreticulin expression in H9c2 cells. Molecular Medicine Reports, 12, 5197-5202. https://doi.org/10.3892/mmr.2015.4020
MLA
Liu, M., Zhang, Y., Lin, X., He, J., Tan, T., Wu, S., Yu, S., Chen, L., Chen, Y., Fu, H., Yuan, C., Li, J."Hydrogen sulfide attenuates doxorubicin‑induced cardiotoxicity by inhibiting calreticulin expression in H9c2 cells". Molecular Medicine Reports 12.4 (2015): 5197-5202.
Chicago
Liu, M., Zhang, Y., Lin, X., He, J., Tan, T., Wu, S., Yu, S., Chen, L., Chen, Y., Fu, H., Yuan, C., Li, J."Hydrogen sulfide attenuates doxorubicin‑induced cardiotoxicity by inhibiting calreticulin expression in H9c2 cells". Molecular Medicine Reports 12, no. 4 (2015): 5197-5202. https://doi.org/10.3892/mmr.2015.4020