Inhibitory effect of 2‑mercaptoethane sulfonate on the formation of Escherichia coli biofilms in vitro

Chen,Sheng; He,Nianhai; Yu,Jialin; Li,Luquan; Sun,Fengjun; Hu,Ying; Deng,Rui; Zhong,Shiming; Shen,Leilei

doi:10.3892/mmr.2015.4112

Inhibitory effect of 2‑mercaptoethane sulfonate on the formation of Escherichia coli biofilms in vitro

Authors:
- Sheng Chen
- Nianhai He
- Jialin Yu
- Luquan Li
- Fengjun Sun
- Ying Hu
- Rui Deng
- Shiming Zhong
- Leilei Shen
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Affiliations: Department of Pediatrics, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China, Department of Neonatology, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China, Department of Pharmacy, Southwest Hospital of The Third Military Medical University, Chongqing 400038, P.R. China
Published online on: July 22, 2015 https://doi.org/10.3892/mmr.2015.4112
Pages: 5223-5230

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Abstract

The biofilms (BF) formed by Escherichia coli (E. coli) is an important cause of chronic and recurrent infections due to its capacity to persist on medical surfaces and indwelling devices, demonstrating the importance of inhibiting the formation of E. coli BF and reducing BF infection. Although 2‑mercaptoethane sulfonate (MESNA) exhibits a marked mucolytic effect clinically, the effect of MESNA on the inhibition of E. coli BF formation remains to be elucidated. The present study investigated whether MESNA inhibits the formation of E. coli BF in vitro. The minimum inhibitory concentration of MESNA on E. coli was determined to be 10 mg/ml. Subsequently, the effect of MESNA on BF early adhesion, extracellular polysaccharide (EPS) and extracellular protein were detected. The effect of a subinhibitory concentration of MESNA on BF formation was evaluated, and the inhibitory potency of MESNA against matured BF was assayed. The results revealed that MESNA inhibited early stage adhesion and formation of the E. coli BF, destroyed the mature BF membrane and reduced the EPS and extracellular proteins levels of the BF. In addition, the present study investigated the effects of MESNA on the expression of EPS‑ and adhesion protein‑associated genes using quantitative polymerase chain reaction analysis, which demonstrated that MESNA effectively inhibited the expression of these genes. These results suggested that MESNA possesses anti‑BF formation capability on E. coli in vitro and may be used as a potential reagent for the clinical treatment of E. coli BF‑associated infections.

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1	Arciola CR: New concepts and new weapons in implant infections. Int J Artif Organs. 32:533–536. 2009.PubMed/NCBI
2	Vasilev K, Cook J and Griesser HJ: Antibacterial surfaces for biomedical devices. Expert Rev Med Devices. 6:553–567. 2009. View Article : Google Scholar : PubMed/NCBI
3	Høiby N, Bjarnsholt T, Givskov M, Molin S and Ciofu O: Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents. 35:322–332. 2010. View Article : Google Scholar : PubMed/NCBI
4	Lopes SP, Ceri H, Azevedo NF and Pereira MO: Antibiotic resistance of mixed biofilms in cystic fibrosis: Impact of emerging microorganisms on treatment of infection. Int J Antimicrob Agents. 40:260–263. 2012. View Article : Google Scholar : PubMed/NCBI
5	Tao XB, Qian LH, Li Y, Wu Q, Ruan JJ, Cai DZ and Peng H: Hospital-acquired infection rate in a tertiary care teaching hospital in China: A cross-sectional survey involving 2434 inpatients. Int J Infect Dis. 27:7–9. 2014. View Article : Google Scholar : PubMed/NCBI
6	Anderson GG, Palermo JJ, Schilling JD, Roth R, Heuser J and Hultgren SJ: Intracellular bacterial biofilm-like pods in urinary tract infections. Science. 301:105–107. 2003. View Article : Google Scholar : PubMed/NCBI
7	Berry RE, Klumpp DJ and Schaeffer AJ: Urothelial cultures support intracellular bacterial community formation by uropathogenic Escherichia coli. Infect Immun. 77:2762–2772. 2009. View Article : Google Scholar : PubMed/NCBI
8	Zhao T and Liu Y: N-acetylcysteine inhibit biofilms produced by Pseudomonas aeruginosa. BMC Microbiol. 10:1402010. View Article : Google Scholar : PubMed/NCBI
9	Marchese A, Bozzolasco M, Gualco L, Debbia EA, Schito GC and Schito AM: Effect of fosfomycin alone and in combination with N-acetylcysteine on E. coli biofilms. Int J Antimicrob Agents. 22:95–100. 2003. View Article : Google Scholar : PubMed/NCBI
10	Leite B, Gomes F, Teixeira P, Souza C, Pizzolitto E and Oliveira R: Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin. Am J Ther. 20:322–328. 2013. View Article : Google Scholar
11	Wu X, Wang Y and Tao L: Sulfhydryl compounds reduce Staphylococcus aureus biofilm formation by inhibiting PIA biosynthesis. FEMS Microbiol Lett. 316:44–50. 2011. View Article : Google Scholar : PubMed/NCBI
12	Lu Q, Yu J, Yang X, Wang J, Wang L, Lin Y and Lin L: Ambroxol interferes with Pseudomonas aeruginosa quorum sensing. Int J Antimicrob Agents. 36:211–215. 2010. View Article : Google Scholar : PubMed/NCBI
13	Li F, Wang W, Hu L, Li L and Yu J: Effect of ambroxol on pneumonia caused by Pseudomonas aeruginosa with biofilm formation in an endotracheal intubation rat model. Chemotherapy. 57:173–180. 2011. View Article : Google Scholar : PubMed/NCBI
14	Kyung YS, Park HY and Lee G: Preservation of uroplakins by 2-mercaptoethanesulfonate in cyclophosphamide-induced rat cystitis. Arch Toxicol. 85:51–57. 2011. View Article : Google Scholar
15	Casale M, Di Martino A, Salvinelli F, Trombetta M and Denaro V: MESNA for chemically assisted tissue dissection. Expert Opin Investig Drugs. 19:699–707. 2010. View Article : Google Scholar : PubMed/NCBI
16	Ludwig U, Riedel MK, Backes M, Imhof A, Muche R and Keller F: MESNA (sodium 2-mercaptoethanesulfonate) for prevention of contrast medium-induced nephrotoxicity-controlled trial. Clin Nephrol. 75:302–308. 2011. View Article : Google Scholar : PubMed/NCBI
17	Tekeres M, Horváth A, Bárdosi L and Kenyeres P: Clinical studies on the mucolytic effect of mesna. Clin Ther. 4:56–60. 1981.PubMed/NCBI
18	Hou S, Liu Z, Young AW, Mark SL, Kallenbach NR and Ren D: Effects of Trp- and Arg-containing antimicrobial-peptide structure on inhibition of Escherichia coli planktonic growth and biofilm formation. Appl Environ Microbiol. 76:1967–1974. 2010. View Article : Google Scholar : PubMed/NCBI
19	Evliyaoğlu Y, Kobaner M, Celebi H, Yelsel K and Doğan A: The efficacy of a novel antibacterial hydroxyapatite nanoparticle-coated indwelling urinary catheter in preventing biofilm formation and catheter-associated urinary tract infection in rabbits. Urol Res. 39:443–449. 2011. View Article : Google Scholar
20	Tran N and Tran PA: Nanomaterial-based treatments for medical device-associated infections. Chemphyschem. 13:2481–2494. 2012. View Article : Google Scholar : PubMed/NCBI
21	Goren MP, Houle JM, Bush DA, Li JT, Newman CE and Brade WP: Similar bioavailability of single-dose oral and intravenous mesna in the blood and urine of healthy human subjects. Clin Cancer Res. 4:2313–2320. 1998.PubMed/NCBI
22	Flemming HC and Wingender J: The biofilm matrix. Nat Rev Microbiol. 8:623–633. 2010.PubMed/NCBI
23	Wayne PA: Clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing: Eighteenth informational supplement. (CLSI document M100-S18). USA: 2008
24	Wu B, Wang Y, Lee YH, Horst A, Wang Z, Chen DR, Sureshkumar R and Tang YJ: Comparative eco-toxicities of nano-ZnO particles under aquatic and aerosol exposure modes. Environ Sci Technol. 44:1484–1489. 2010. View Article : Google Scholar : PubMed/NCBI
25	Saha SK and Brewer CF: Determination of the concentrations of oligosaccharides, complex type carbohydrates and glycoproteins using the phenol-sulfuric acid method. Carbohydr Res. 254:157–167. 1994. View Article : Google Scholar : PubMed/NCBI
26	Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254. 1976. View Article : Google Scholar : PubMed/NCBI
27	Strathmann M, Wingender J and Flemming HC: Application of fluorescently labelled lectins for the visualization and biochemical characterization of polysaccharides in biofilms of Pseudomonas aeruginosa. J Microbiol Methods. 50:237–248. 2002. View Article : Google Scholar : PubMed/NCBI
28	Beyenal H, Donova C, Lewandowski Z and Harkin G: Three-dimensional biofilm structure quantification. J Microbiol Methods. 59:395–413. 2004. View Article : Google Scholar : PubMed/NCBI
29	Ulett GC, Valle J, Beloin C, Sherlock O, Ghigo JM and Schembri MA: Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract. Infect Immun. 75:3233–3244. 2007. View Article : Google Scholar : PubMed/NCBI
30	Naves P, del Prado G, Huelves L, Gracia M, Ruiz V, Blanco J, Dahbi G, Blanco M, Ponte Mdel C and Soriano F: Correlation between virulence factors and in vitro biofilm formation by Escherichia coli strains. Microb Pathog. 45:86–91. 2008. View Article : Google Scholar : PubMed/NCBI
31	Schembri MA and Klemm P: Biofilm formation in a hydrodynamic environment by novel fimh variants and ramifications for virulence. Infect Immun. 69:1322–1328. 2001. View Article : Google Scholar : PubMed/NCBI
32	Tiba MR, Nogueira GP and Leite Dda S: Study on virulence factors associated with biofilm formation and phylogenetic groupings in Escherichia coli strains isolated from patients with cystitis. Rev Soc Bras Med Trop. 42:58–62. 2009.In Portuguese. View Article : Google Scholar : PubMed/NCBI
33	Itoh Y, Rice JD, Goller C, Pannuri A, Taylor J, Meisner J, Beveridge TJ, Preston JF III and Romeo T: Roles of pgaABCD genes in synthesis, modification and export of the Escherichia coli biofilm adhesin polybeta-1,6- N-acetyl-d-gluc osamine. J Bacteriol. 190:3670–3680. 2008. View Article : Google Scholar : PubMed/NCBI
34	Suman E, D'souza SJ, Jacob P, Sushruth MR and Kotian MS: Anti-biofilm and anti-adherence activity of Glm-U inhibitors. Indian J Med Sc. 65:387–392. 2011. View Article : Google Scholar
35	Yeom J, Lee Y and Park W: Effects of non-ionic solute stresses on biofilm formation and lipopolysaccharide production in Escherichia coli O157H7. Res Microbiol. 163:258–267. 2012. View Article : Google Scholar : PubMed/NCBI
36	Ulaganathan V, Buetow L and Hunter WN: Nucleotide substrate recognition by UDP-N-acetylglucosamine acyltransferase (LpxA) in the first step of lipid A biosynthesis. J Mol Biol. 369:305–312. 2007. View Article : Google Scholar : PubMed/NCBI