Endosialin‑expressing bone sarcoma stem‑like cells are highly tumor‑initiating and invasive

Sun,Dong‑Xiu; Liao,Guang‑Jun; Liu,Ke‑Gui; Jian,Han

doi:10.3892/mmr.2015.4218

Endosialin‑expressing bone sarcoma stem‑like cells are highly tumor‑initiating and invasive

Authors:
- Dong‑Xiu Sun
- Guang‑Jun Liao
- Ke‑Gui Liu
- Han Jian
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Affiliations: Department of Operating Room, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China, Department of Orthopaedic Surgery, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China, The Joint Department of Orthopaedics, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China
Published online on: August 12, 2015 https://doi.org/10.3892/mmr.2015.4218
Pages: 5665-5670
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It has been reported that the presence of a small group of cancer stem‑like ‘side population (SP)’ cells is responsible for therapy failure and tumor recurrence. The present study demonstrated that primary human osteosarcoma samples contained a SP of about 3.9% which overexpressed ABC transporters, including ABCA1, ABCB1, ABCB2 and ABCG2, which are associated with drug resistance and may have contributed to multi‑drug resistance of SP cells. Furthermore, these SP cells displayed increased expression of endosialin (CD248) and other stem cell surface proteins, including CD133, octamer‑binding transcription factor 3/4A, Nanog and Nestin, which are ultimately responsible for high self‑renewal and deregulated cell proliferation. In addition, it was shown that endosialin‑overexpressing SP cells were able to regenerate the tumor population and had a high invasive potential. Therefore, the present study suggested that osteosarcoma SP cells were cancer stem cells, as they displayed stem‑like properties; furthermore, endosialin may be a potential target to prevent osteosarcoma recurrence following chemotherapy.

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