Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells

Ma,Ping; Gao,Qianying; Wang,Zhichong; Yu,Keming

doi:10.3892/mmr.2015.4205

Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells

Authors:
- Ping Ma
- Qianying Gao
- Zhichong Wang
- Keming Yu
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Affiliations: Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen University, Guangzhou, Guangdong 510060, P.R. China
Published online on: August 10, 2015 https://doi.org/10.3892/mmr.2015.4205
Pages: 6025-6030

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Abstract

Members of the protein kinase C (PKC) family are involved in physiological and pathophysiological processes, and exert an important role in signal transduction. The aim of the present study was to determine which of the 12 protein kinase C (PKC) isoforms (PKCα, PKCβⅠ, PKCβⅡ, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCµ, PKCζ, PKCλ and PKCι) were expressed in vitro in cultured human Tenon's capsule fibroblasts (HTFs). HTFs from cell passages three to five were investigated for the presence of the 12 PKC isoforms at the cellular, mRNA and protein levels using laser scanning confocal microscopy (LSCM), reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis, respectively. These analyses yielded similar results for several of the PKC isoforms (PKCα, PKCδ, PKCε, PKCη, PKCµ, PKCζ, PKCλ and PKCι); however, PKCβⅠ, PKCβⅡ, PKCγ and PKCθ were not expressed in the cultured HTFs. LSCM revealed that eight PKC isoforms, PKCα, PKCδ, PKCε, PKCη, PKCµ, PKCζ, PKCλ and PKCι, appeared almost exclusively in the cytoplasm of the cells. Notably, PKCδ was expressed particularly well in the cytoskeleton. The present study revealed that all 12 PKC isoforms were expressed and that eight of the isoforms were present in cultured HTFs. These results will be of value when determining specific roles for the PKC isoforms in HTF proliferation, which may provide a novel therapeutic target for bleb scarring in glaucoma filtering surgery.

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