MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells

  • Authors:
    • Hongyan Huang
    • Yueyong Zhu
    • Shaoyang Li
  • View Affiliations

  • Published online on: August 26, 2015     https://doi.org/10.3892/mmr.2015.4254
  • Pages: 6918-6924
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Abstract

There is currently a requirement for effective treatment strategies for human hepatocellular carcinoma (HCC), a leading cause of cancer‑associated mortality. MicroRNA-122 (miR-122), a repressor of the endogenous apoptosis regulator Bcl‑w, is frequently downregulated in HCC. Thus, it is hypothesized that the activation of miR‑122 may induce selective hepatocellular apoptosis via caspase activation in a model of HCC. In the present study, an miR‑122 mimic transfection was performed in HepG2 cells, and used to investigate the role and therapeutic potential of miR‑122 in the regulation of HCC‑derived cell lines. The apoptotic rates of HepG2 cells were significantly increased following miR‑122 mimic transfection. Reverse transcription‑polymerase chain reaction analysis revealed that Bcl‑w mRNA was significantly reduced, while the mRNA levels of caspase‑9 and caspase‑3 were markedly increased. The immunocytochemistry results supported the mRNA trends. Collectively, the present results suggest that endogenous miR‑122 contributes to HepG2 apoptosis and that transfection of mimic miR‑122 normalizes apoptotic levels in a model of HCC.
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November-2015
Volume 12 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Huang H, Zhu Y and Li S: MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells. Mol Med Rep 12: 6918-6924, 2015.
APA
Huang, H., Zhu, Y., & Li, S. (2015). MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells. Molecular Medicine Reports, 12, 6918-6924. https://doi.org/10.3892/mmr.2015.4254
MLA
Huang, H., Zhu, Y., Li, S."MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells". Molecular Medicine Reports 12.5 (2015): 6918-6924.
Chicago
Huang, H., Zhu, Y., Li, S."MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells". Molecular Medicine Reports 12, no. 5 (2015): 6918-6924. https://doi.org/10.3892/mmr.2015.4254