Taspine derivative TAS9 regulates cell growth and metastasis of human hepatocellular carcinoma

  • Authors:
    • Rui Liu
    • Wenjie Wang
    • Bingling Dai
    • Yanping Liu
    • Yanmin Zhang
  • View Affiliations

  • Published online on: September 30, 2015     https://doi.org/10.3892/mmr.2015.4398
  • Pages: 7735-7741
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Taspine has been indicated to be a potential anti‑carcinogenic agent. The present study investigated the effects of TAS9, a modified taspine derivative, on the proliferation and migration of the SMMC‑7721 human liver cancer cell line. First, the effects of TAS9 on SMMC‑7721 cell growth were examined using MTT and colony formation assaya. In vivo Transwell and wound healing assays were then performed to assess the inhibitory effects of TAS9 on cell invasion and migration, respectively. The expression of cell proliferation‑ and migration‑associated signaling molecules was investigated by western blot analysis. The results indicated that TAS9 inhibited SMMC‑7721 cell growth by downregulating the signaling molecules protein kinase Cβ (PKCβ), Akt, mammalian target of rapamycin, mitogen‑activated protein kinase kinase 2, RAF and c‑Jun N‑terminal kinase‑1, and inhibiting SMMC‑7721 cell migration by suppressing the expression of matrix metalloproteinase (MMP)‑2, MMP‑9, chemokine (C‑X‑C motif) receptor 4, nuclear factor κB, p38 and p53. Small interfering RNA‑mediated knockdown of PKCβ in the SMMC‑7721 cells significantly attenuated the tumor inhibitory effects of TAS9. In conclusion, the results of the present study suggested that TAS9 may have inhibitory effects on the proliferation and migration of SMMC‑7721 cells, and may serve as a potential candidate for cancer treatment.
View Figures
View References

Related Articles

Journal Cover

November-2015
Volume 12 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu R, Wang W, Dai B, Liu Y and Zhang Y: Taspine derivative TAS9 regulates cell growth and metastasis of human hepatocellular carcinoma. Mol Med Rep 12: 7735-7741, 2015.
APA
Liu, R., Wang, W., Dai, B., Liu, Y., & Zhang, Y. (2015). Taspine derivative TAS9 regulates cell growth and metastasis of human hepatocellular carcinoma. Molecular Medicine Reports, 12, 7735-7741. https://doi.org/10.3892/mmr.2015.4398
MLA
Liu, R., Wang, W., Dai, B., Liu, Y., Zhang, Y."Taspine derivative TAS9 regulates cell growth and metastasis of human hepatocellular carcinoma". Molecular Medicine Reports 12.5 (2015): 7735-7741.
Chicago
Liu, R., Wang, W., Dai, B., Liu, Y., Zhang, Y."Taspine derivative TAS9 regulates cell growth and metastasis of human hepatocellular carcinoma". Molecular Medicine Reports 12, no. 5 (2015): 7735-7741. https://doi.org/10.3892/mmr.2015.4398