MicroRNA-519 enhances HL60 human acute myeloid leukemia cell line proliferation by reducing the expression level of RNA-binding protein human antigen R

Huang,Kebin; Dong,Bingwei; Wang,Yueyue; Tian,Tao; Zhang,Biying

doi:10.3892/mmr.2015.4455

MicroRNA-519 enhances HL60 human acute myeloid leukemia cell line proliferation by reducing the expression level of RNA-binding protein human antigen R

Authors:
- Kebin Huang
- Bingwei Dong
- Yueyue Wang
- Tao Tian
- Biying Zhang
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Affiliations: Nuclear Medicine Department, The Third Hospital of Chinese People's Liberation Army, Baoji, Shanxi 721004, P.R. China, Department of Pathology, The Central Hospital of Xianyang, Xianyang, Shaanxi 710065, P.R. China, Department of Pathology, The Second Affiliated Hospital of Medicine College, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 100044, P.R. China, Clinical Laboratory, People's Hospital of Tongchuan, Tongchuan, Shaanxi 727500, P.R. China
Published online on: October 20, 2015 https://doi.org/10.3892/mmr.2015.4455
Pages: 7830-7836
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have demonstrated that microRNAs (miRs) are involved in cell apoptosis. However, the role of miR-519 in acute myeloid leukemia (AML) has yet to be elucidated. The present study identified the effects of miR‑519 on HL60 human acute myeloid leukemia cell growth and apoptosis. The expression levels of miR‑519 were examined in AML cells, as well as AML tissue samples. Furthermore, cell viability and apoptosis were examined in HL60 cells transfected with miR‑519 mimics, miR‑519 inhibitors or a negative control. In addition, the effects of human antigen R (HuR) on cell apoptosis were investigated using specific small interfering RNA targeting HuR. The results demonstrated that the expression levels of miR‑519 were significantly increased in the AML cells and the tissue samples, suggesting that miR‑519 may contribute to abnormal HL60 cell proliferation. Upregulation of miR‑519 expression decreased HL60 cell viability and induced cell apoptosis. Furthermore, knockdown of HuR reduced cell migration and enhanced cell apoptosis. The results of the present study indicate that miR‑519 may contribute to HL60 cell apoptosis by regulating the expression of HuR.

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